6QFA

CryoEM structure of a beta3K279T GABA(A)R homomer in complex with histamine and megabody Mb25

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, G, H, I, J (B, C, D, E, A)	Gamma-aminobutyric acid receptor subunit beta-3,Gamma-aminobutyric acid receptor subunit beta-3	polymer	341	39503.6	5	UniProt (P28472) Pfam (PF02931) Pfam (PF02932)	Homo sapiens (Human)	GABA(A) receptor subunit beta-3,GABA(A) receptor subunit beta-3
2	B, C, D, E, F (K, O, N, M, L)	Outer membrane protein,Outer membrane protein,Outer membrane protein,Outer membrane protein,Uncharacterized protein,Uncharacterized protein,Mb-c7HopQ-Nb25	polymer	522	56300.6	5	UniProt (B5Z8H1) Pfam (PF18304)	Helicobacter pylori (strain G27)
3	K, L, M, N, O (F, G, H, I, J)	alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		910.8	5	In PDB GlyTouCan (G22768VO)
4	P, R, T, V, X (B, C, D, E, A)	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	5	Chemie (NAG)
5	Q, S, U, W, Y (B, C, D, E, A)	HISTAMINE	non-polymer		111.1	5	Chemie (HSM)

Sequence modifications

B, C, D, E, A: 1 - 307 (UniProt: P28472)

PDB	External Database	Details
Thr 279	Lys 304	engineered mutation

B, C, D, E, A: 422 - 448 (UniProt: P28472)

K, O, N, M, L: 13 - 233 (UniProt: B5Z8H1)

K, O, N, M, L: 234 - 402 (UniProt: B5Z8H1)
K, O, N, M, L: 403 - 522 (PDB: 6QFA)

Sequence viewer

Contents of the asymmetric unit

*Water molecules are not included.