6QFA
CryoEM structure of a beta3K279T GABA(A)R homomer in complex with histamine and megabody Mb25
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | B, C, D, E, A | Gamma-aminobutyric acid receptor subunit beta-3,Gamma-aminobutyric acid receptor subunit beta-3 | polymer | 341 | 39503.6 | 5 | UniProt (P28472) Pfam (PF02931) Pfam (PF02932) In PDB | Homo sapiens (Human) | GABA(A) receptor subunit beta-3,GABA(A) receptor subunit beta-3 |
| 2 | K, O, N, M, L | Outer membrane protein,Outer membrane protein,Outer membrane protein,Outer membrane protein,Uncharacterized protein,Uncharacterized protein,Mb-c7HopQ-Nb25 | polymer | 522 | 56300.6 | 5 | UniProt (B5Z8H1) Pfam (PF18304) In PDB | Helicobacter pylori (strain G27) | |
| 3 | F, G, H, I, J | alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 910.8 | 5 | ||||
| 4 | B, C, D, E, A | 2-acetamido-2-deoxy-beta-D-glucopyranose | non-polymer | 221.2 | 5 | Chemie (NAG) | |||
| 5 | B, C, D, E, A | HISTAMINE | non-polymer | 111.1 | 5 | Chemie (HSM) |
Sequence modifications
B, C, D, E, A: 1 - 307 (UniProt: P28472)
B, C, D, E, A: 422 - 448 (UniProt: P28472)
K, O, N, M, L: 13 - 233 (UniProt: B5Z8H1)
K, O, N, M, L: 234 - 402 (UniProt: B5Z8H1)
K, O, N, M, L: 403 - 522 (PDB: 6QFA)
| PDB | External Database | Details |
|---|---|---|
| Thr 279 | Lys 304 | engineered mutation |
| PDB | External Database | Details |
|---|---|---|
| Ser 308 | - | linker |
| Gln 309 | - | linker |
| Pro 310 | - | linker |
| Ala 311 | - | linker |
| Arg 312 | - | linker |
| Ala 313 | - | linker |
| Ala 314 | - | linker |
| PDB | External Database | Details |
|---|---|---|
| Gln 1 | - | expression tag |
| Val 2 | - | expression tag |
| Gln 3 | - | expression tag |
| Leu 4 | - | expression tag |
| Val 5 | - | expression tag |
| Glu 6 | - | expression tag |
| Ser 7 | - | expression tag |
| Gly 8 | - | expression tag |
| Gly 9 | - | expression tag |
| Gly 10 | - | expression tag |
| Leu 11 | - | expression tag |
| Val 12 | - | expression tag |
K, O, N, M, L: 403 - 522 (PDB: 6QFA)
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 10 |
| Total formula weight | 479020.9 | |
| Branched | Number of molecules | 5 |
| Total formula weight | 4554.1 | |
| Non-Polymers* | Number of molecules | 10 |
| Total formula weight | 1661.8 | |
| All* | Total formula weight | 485236.8 |
