Summary Structural details Experimental details Functional details Sequence Neighbor History Downloads

2BCJ

Crystal Structure of G Protein-Coupled Receptor Kinase 2 in Complex with Galpha-q and Gbetagamma Subunits

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0001664	molecular_function	G protein-coupled receptor binding
A	0002026	biological_process	regulation of the force of heart contraction
A	0002029	biological_process	desensitization of G protein-coupled receptor signaling pathway
A	0002031	biological_process	G protein-coupled receptor internalization
A	0003108	biological_process	negative regulation of the force of heart contraction by chemical signal
A	0004672	molecular_function	protein kinase activity
A	0004674	molecular_function	protein serine/threonine kinase activity
A	0004703	molecular_function	G protein-coupled receptor kinase activity
A	0005515	molecular_function	protein binding
A	0005524	molecular_function	ATP binding
A	0005737	cellular_component	cytoplasm
A	0005829	cellular_component	cytosol
A	0005886	cellular_component	plasma membrane
A	0006468	biological_process	protein phosphorylation
A	0006886	biological_process	intracellular protein transport
A	0007165	biological_process	signal transduction
A	0007186	biological_process	G protein-coupled receptor signaling pathway
A	0007213	biological_process	G protein-coupled acetylcholine receptor signaling pathway
A	0009966	biological_process	regulation of signal transduction
A	0016020	cellular_component	membrane
A	0018105	biological_process	peptidyl-serine phosphorylation
A	0031694	molecular_function	alpha-2A adrenergic receptor binding
A	0031755	molecular_function	Edg-2 lysophosphatidic acid receptor binding
A	0042995	cellular_component	cell projection
A	0045202	cellular_component	synapse
A	0045880	biological_process	positive regulation of smoothened signaling pathway
A	0045988	biological_process	negative regulation of striated muscle contraction
A	0047696	molecular_function	beta-adrenergic receptor kinase activity
A	0060048	biological_process	cardiac muscle contraction
A	0098793	cellular_component	presynapse
A	0098794	cellular_component	postsynapse
A	1901081	biological_process	negative regulation of relaxation of smooth muscle
A	1903566	biological_process	positive regulation of protein localization to cilium
B	0005515	molecular_function	protein binding
B	0005737	cellular_component	cytoplasm
B	0005834	cellular_component	heterotrimeric G-protein complex
B	0007165	biological_process	signal transduction
B	0007186	biological_process	G protein-coupled receptor signaling pathway
B	0007191	biological_process	adenylate cyclase-activating dopamine receptor signaling pathway
B	0016020	cellular_component	membrane
B	0030159	molecular_function	signaling receptor complex adaptor activity
B	0071380	biological_process	cellular response to prostaglandin E stimulus
B	0071870	biological_process	cellular response to catecholamine stimulus
B	0097381	cellular_component	photoreceptor disc membrane
G	0003924	molecular_function	GTPase activity
G	0005515	molecular_function	protein binding
G	0005834	cellular_component	heterotrimeric G-protein complex
G	0005886	cellular_component	plasma membrane
G	0007165	biological_process	signal transduction
G	0007186	biological_process	G protein-coupled receptor signaling pathway
G	0007191	biological_process	adenylate cyclase-activating dopamine receptor signaling pathway
G	0016020	cellular_component	membrane
G	0031681	molecular_function	G-protein beta-subunit binding
G	0071380	biological_process	cellular response to prostaglandin E stimulus
G	0071870	biological_process	cellular response to catecholamine stimulus
Q	0001501	biological_process	skeletal system development
Q	0001508	biological_process	action potential
Q	0001664	molecular_function	G protein-coupled receptor binding
Q	0003924	molecular_function	GTPase activity
Q	0003925	molecular_function	G protein activity
Q	0005096	molecular_function	GTPase activator activity
Q	0005515	molecular_function	protein binding
Q	0005525	molecular_function	GTP binding
Q	0005634	cellular_component	nucleus
Q	0005737	cellular_component	cytoplasm
Q	0005794	cellular_component	Golgi apparatus
Q	0005834	cellular_component	heterotrimeric G-protein complex
Q	0005886	cellular_component	plasma membrane
Q	0005901	cellular_component	caveola
Q	0007165	biological_process	signal transduction
Q	0007186	biological_process	G protein-coupled receptor signaling pathway
Q	0007189	biological_process	adenylate cyclase-activating G protein-coupled receptor signaling pathway
Q	0007200	biological_process	phospholipase C-activating G protein-coupled receptor signaling pathway
Q	0007207	biological_process	phospholipase C-activating G protein-coupled acetylcholine receptor signaling pathway
Q	0007215	biological_process	glutamate receptor signaling pathway
Q	0007507	biological_process	heart development
Q	0008217	biological_process	regulation of blood pressure
Q	0009791	biological_process	post-embryonic development
Q	0010543	biological_process	regulation of platelet activation
Q	0016020	cellular_component	membrane
Q	0016322	biological_process	neuron remodeling
Q	0019001	molecular_function	guanyl nucleotide binding
Q	0021884	biological_process	forebrain neuron development
Q	0030234	molecular_function	enzyme regulator activity
Q	0030425	cellular_component	dendrite
Q	0031683	molecular_function	G-protein beta/gamma-subunit complex binding
Q	0031965	cellular_component	nuclear membrane
Q	0032024	biological_process	positive regulation of insulin secretion
Q	0042711	biological_process	maternal behavior
Q	0042733	biological_process	embryonic digit morphogenesis
Q	0043066	biological_process	negative regulation of apoptotic process
Q	0043267	biological_process	negative regulation of potassium ion transport
Q	0044297	cellular_component	cell body
Q	0044877	molecular_function	protein-containing complex binding
Q	0045202	cellular_component	synapse
Q	0045634	biological_process	regulation of melanocyte differentiation
Q	0046872	molecular_function	metal ion binding
Q	0047391	molecular_function	alkylglycerophosphoethanolamine phosphodiesterase activity
Q	0048066	biological_process	developmental pigmentation
Q	0048661	biological_process	positive regulation of smooth muscle cell proliferation
Q	0060158	biological_process	phospholipase C-activating dopamine receptor signaling pathway
Q	0086100	biological_process	endothelin receptor signaling pathway
Q	0099105	biological_process	ion channel modulating, G protein-coupled receptor signaling pathway
Q	1904888	biological_process	cranial skeletal system development
Q	1990806	biological_process	ligand-gated ion channel signaling pathway

Functional Information from PDB Data

site_id	AC1
Number of Residues	6
Details	BINDING SITE FOR RESIDUE MG Q 361

Chain	Residue
Q	SER53
Q	THR186
Q	GDP360
Q	ALF362
Q	HOH364
Q	HOH365

site_id	AC2
Number of Residues	14
Details	BINDING SITE FOR RESIDUE ALF Q 362

Chain	Residue
Q	ARG183
Q	PRO185
Q	THR186
Q	VAL206
Q	GLY208
Q	GLN209
Q	GDP360
Q	MG361
Q	HOH363
Q	HOH364
Q	HOH365
Q	GLY48
Q	GLU49
Q	LYS52

site_id	AC3
Number of Residues	24
Details	BINDING SITE FOR RESIDUE GDP Q 360

Chain	Residue
Q	GLU49
Q	SER50
Q	GLY51
Q	LYS52
Q	SER53
Q	THR54
Q	ASP155
Q	SER156
Q	LEU180
Q	ARG181
Q	VAL182
Q	ARG183
Q	ASN274
Q	LYS275
Q	ASP277
Q	LEU278
Q	CYS330
Q	ALA331
Q	THR332
Q	MG361
Q	ALF362
Q	HOH364
Q	HOH365
Q	HOH367

Functional Information from PROSITE/UniProt

site_id	PS00107
Number of Residues	24
Details	PROTEIN_KINASE_ATP Protein kinases ATP-binding region signature. IGRGGFGEVYgCrkadtgkm..........YAMK

Chain	Residue	Details
A	ILE197-LYS220

site_id	PS00108
Number of Residues	13
Details	PROTEIN_KINASE_ST Serine/Threonine protein kinases active-site signature. VvYrDLKpaNILL

Chain	Residue	Details
A	VAL313-LEU325

site_id	PS00678
Number of Residues	15
Details	WD_REPEATS_1 Trp-Asp (WD) repeats signature. LVSAsqDgKLIIWDS

Chain	Residue	Details
B	LEU70-SER84
B	ILE157-ILE171
B	LEU285-ALA299

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	1
Details	LIPID: N-myristoyl glycine => ECO:0000269\|PubMed:26253820

Chain	Residue	Details
Q	GLY8

site_id	SWS_FT_FI2
Number of Residues	1
Details	LIPID: S-palmitoyl cysteine => ECO:0000269\|PubMed:26253820

Chain	Residue	Details
Q	CYS9
A	LYS220

site_id	SWS_FT_FI3
Number of Residues	1
Details	BINDING: BINDING => ECO:0000250\|UniProtKB:P29992

Chain	Residue	Details
Q	GLY46
A	LEU4
A	ASP10

site_id	SWS_FT_FI4
Number of Residues	5
Details	BINDING: BINDING => ECO:0000250\|UniProtKB:P27600

Chain	Residue	Details
Q	SER53
Q	LEU180
Q	THR186
Q	ASN274
Q	ALA331

site_id	SWS_FT_FI5
Number of Residues	1
Details	MOD_RES: 5-glutamyl histamine => ECO:0000269\|PubMed:23022564

Chain	Residue	Details
Q	GLN209

Catalytic Information from CSA

site_id	MCSA1
Number of Residues
Details	M-CSA 533

Chain

Residue

Details

site_id	CSA1
Number of Residues	4
Details	Annotated By Reference To The Literature 1ir3

Chain	Residue	Details
Q	THR186
Q	GLN209
Q	GLU49
Q	ARG183

site_id	CSA2
Number of Residues	2
Details	Annotated By Reference To The Literature 1ir3

Chain	Residue	Details
A	ASP317
A	ALA321

site_id	CSA3
Number of Residues	2
Details	Annotated By Reference To The Literature 1ir3

Chain	Residue	Details
A	ASP317
A	LYS319

site_id	CSA4
Number of Residues	1
Details	Annotated By Reference To The Literature 1ir3

Chain	Residue	Details
Q	GLN209

site_id	CSA5
Number of Residues	3
Details	Annotated By Reference To The Literature 1ir3

Chain	Residue	Details
A	THR353
A	ASP317
A	LYS319

site_id	CSA6
Number of Residues	3
Details	Annotated By Reference To The Literature 1ir3

Chain	Residue	Details
A	ASP317
A	LYS319
A	ASN322

		Sequence (fasta)
		PDBx/mmCIF
		PDBML format (no-atom)
		PDB format (full)
		Validation report (PDF)
		EDMap 2fo-fc (MTZ)