8UKS

RNA polymerase II elongation complex with Fapy-dG lesion soaking with CTP before chemistry

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.1
Synchrotron site	ALS
Beamline	5.0.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-03-23
Detector	DECTRIS PILATUS3 2M
Wavelength(s)	0.9774
Spacegroup name	C 1 2 1
Unit cell lengths	157.118, 221.626, 189.961
Unit cell angles	90.00, 97.40, 90.00

Refinement procedure

Resolution	47.760 - 3.400
R-factor	0.2675
Rwork	0.267
R-free	0.30150
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.831
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.760	3.450
High resolution limit [Å]	3.390	3.390
Rmerge	0.524	4.497
Rmeas	0.566	4.874
Rpim	0.214	1.858
Total number of observations	615254	30346
Number of reflections	89064	4492
<I/σ(I)>	4.6	0.6
Completeness [%]	99.9
Redundancy	6.9	6.8
CC(1/2)	0.984	0.350

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		295	390 mM ammonium phosphate (pH 6.0), 5 mM DTT, 5 mM dioxane, and 9-13% (w/v) PEG 6,000