6YR8
Affimer K6 - KRAS protein complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04-1 |
Synchrotron site | Diamond |
Beamline | I04-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-12-03 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.9159 |
Spacegroup name | I 41 |
Unit cell lengths | 71.574, 71.574, 144.390 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 64.130 | 1.950 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.087 | 2.690 |
Rpim | 0.025 | 0.834 |
Number of reflections | 28572 | 2116 |
<I/σ(I)> | 13.3 | 0.6 |
Completeness [%] | 100.0 | 100 |
Redundancy | 13.3 | 11.4 |
CC(1/2) | 1.000 | 0.799 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5 | 291 | 0.1M sodium acetate, 25% w/v PEG 4000, 0.2M ammonium sulfate, 5% MPD |