5XQ0
Structural basis of kindlin-mediated integrin recognition and activation
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U1 |
| Synchrotron site | SSRF |
| Beamline | BL17U1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-03-22 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 75.866, 75.866, 384.219 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 45.858 - 2.750 |
| R-factor | 0.2341 |
| Rwork | 0.232 |
| R-free | 0.28450 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5xpz |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.486 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.8.4_1496) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.800 |
| High resolution limit [Å] | 2.750 | 7.460 | 2.750 |
| Rmerge | 0.086 | 0.048 | 0.788 |
| Rmeas | 0.098 | 0.053 | 0.895 |
| Rpim | 0.045 | 0.022 | 0.413 |
| Total number of observations | 139053 | ||
| Number of reflections | 34443 | ||
| <I/σ(I)> | 12 | ||
| Completeness [%] | 98.7 | 97.5 | 99.8 |
| Redundancy | 4 | 5 | 4.4 |
| CC(1/2) | 0.997 | 0.516 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 289 | 0.2 M potassium chloride, 0.05 M HEPES pH 7.5, 35% v/v pentaerythritol propoxylate |
