5V5S
multi-drug efflux; membrane transport; RND superfamily; Drug resistance
EM specimen
| Vitrification | Yes |
| Instrument | FEI VITROBOT MARK IV |
EM Data Collection
| Microscope | FEI TITAN KRIOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Detector | GATAN K2 SUMMIT (4k x 4k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 6.5 |
| Number of Particle | 95410 |
| Software | RELION,EMAN,PHENIX,Coot,CTFFIND,UCSF Chimera |
| Symmetry | C3 |
| CTF correction | PHASE FLIPPING ONLY |
