5FHA
Crystal Structure of Protective Ebola Virus Antibody 114
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-11-11 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9792 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 171.540, 63.900, 39.130 |
Unit cell angles | 90.00, 101.64, 90.00 |
Refinement procedure
Resolution | 42.117 - 1.973 |
R-factor | 0.1816 |
Rwork | 0.179 |
R-free | 0.23000 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4k8r 3wd5 |
RMSD bond length | 0.005 |
RMSD bond angle | 0.910 |
Data reduction software | MOSFLM |
Data scaling software | Aimless (0.3.11) |
Phasing software | PHASER |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 42.120 | 42.120 | 2.020 |
High resolution limit [Å] | 1.970 | 8.820 | 1.970 |
Rmerge | 0.124 | 0.039 | 0.787 |
Rpim | 0.079 | 0.024 | 0.554 |
Total number of observations | 100911 | 1196 | 6398 |
Number of reflections | 29265 | ||
<I/σ(I)> | 7.4 | 15.2 | 2.1 |
Completeness [%] | 99.9 | 98.9 | 99.8 |
Redundancy | 3.4 | 3.4 | 3 |
CC(1/2) | 0.990 | 0.997 | 0.422 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 5.29mg/mL 114 Fab, 27% PEG 3350, 4.32% isopropanol, 0.1M HEPES pH 7.5, 0.1M calcium chloride, 0.01M sodium bromide |