5E7M

Crystal structure of the active catalytic core of the human DEAD-box protein DDX3 bound to AMPPNP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.3.1
Synchrotron site	ALS
Beamline	8.3.1
Temperature [K]	100
Detector technology	CCD
Collection date	2013-02-03
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.115869
Spacegroup name	P 21 21 21
Unit cell lengths	53.930, 100.280, 107.260
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.497 - 2.304
R-factor	0.2276
Rwork	0.225
R-free	0.28400
RMSD bond length	0.010
RMSD bond angle	1.402
Data reduction software	XDS
Phasing software	PHASER
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	47.500		2.386
High resolution limit [Å]	2.304	10.290	2.304
Rmerge	0.127	0.030	14.820
Rmeas	0.135	0.033	27.419
Total number of observations	198462
Number of reflections	25668	313	1867
<I/σ(I)>	10.8	39.99	0.13
Completeness [%]	97.2	88.4	74.66
Redundancy	7.5		7.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5	291	PEG-3000, sodium citrate