5D9Q
Crystal Structure of the BG505 SOSIP gp140 HIV-1 Env trimer in Complex with the Broadly Neutralizing Fab PGT122 and scFv NIH45-46
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-D |
| Synchrotron site | APS |
| Beamline | 23-ID-D |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-06-22 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.03324 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 153.760, 254.350, 283.550 |
| Unit cell angles | 90.00, 100.96, 90.00 |
Refinement procedure
| Resolution | 39.894 - 4.400 |
| R-factor | 0.2904 |
| Rwork | 0.290 |
| R-free | 0.31960 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4nco |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.804 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 4.500 |
| High resolution limit [Å] | 4.400 | 4.400 |
| Number of reflections | 67706 | |
| <I/σ(I)> | 5.9 | 2 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 20 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 2.4 M ammonium sulfate, 0.1 M Tris, pH 8.0 |
