4WN5

Crystal structure of the C-terminal Per-Arnt-Sim (PASb) of human HIF-3alpha9 bound to 18:1-1-monoacylglycerol

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P13 (MX1)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-09-25
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.977240
Spacegroup name	P 21 2 21
Unit cell lengths	52.650, 53.890, 67.790
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.500 - 1.150
R-factor	0.1226
Rwork	0.122
R-free	0.14080
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4h6j
RMSD bond length	0.011
RMSD bond angle	1.722
Refinement software	PHENIX ((phenix.refine: 1.8.4_1496))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	28.500	1.170
High resolution limit [Å]	1.150	1.150
Number of reflections	68827
<I/σ(I)>	11.5
Completeness [%]	99.6	99.4
Redundancy	9.8	9.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.9	291	3% PEG 400, 1.95 M ammonium sulfate, 0.1 M sodium acetate