4O1P
Crystal Structure of RNase L in complex with 2-5A and AMP-PNP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2013-02-01 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9792 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 59.197, 267.392, 110.210 |
Unit cell angles | 90.00, 90.04, 90.00 |
Refinement procedure
Resolution | 59.200 - 2.500 |
R-factor | 0.1975 |
Rwork | 0.196 |
R-free | 0.23107 |
Structure solution method | MR-SAD |
Starting model (for MR) | 4g8k |
RMSD bond length | 0.007 |
RMSD bond angle | 1.231 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 59.200 | 2.569 |
High resolution limit [Å] | 2.500 | 2.504 |
Rmerge | 0.104 | 0.526 |
Number of reflections | 106347 | |
<I/σ(I)> | 6.8 | 1.6 |
Completeness [%] | 95.7 | 88.4 |
Redundancy | 3 | 3.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 5 mM MgCl2, 5 mM DTT, 18% PEG2000, 100 mM NaCl, and 100 mM SPG buffer, pH 7. , VAPOR DIFFUSION, HANGING DROP, temperature 293K |