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4JV5

Crystal structures of pseudouridinilated stop codons with ASLs

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsDIAMOND BEAMLINE I04
Synchrotron siteDiamond
BeamlineI04
Temperature [K]77
Detector technologyCCD
Collection date2012-08-02
DetectorADSC QUANTUM 315r
Wavelength(s)1.000
Spacegroup nameP 41 21 2
Unit cell lengths401.000, 401.000, 176.000
Unit cell angles90.00, 90.00, 90.00
Refinement procedure
Resolution29.880 - 3.162
R-factor0.20002
Rwork0.197
R-free0.24905
Structure solution methodMOLECULAR REPLACEMENT
RMSD bond length0.009
RMSD bond angle1.698
Data reduction softwareXDS
Data scaling softwareXDS
Phasing softwareMOLREP
Refinement softwareREFMAC (5.8.0016)
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]45.8003.300
High resolution limit [Å]3.1003.100
Number of reflections241499
<I/σ(I)>1.8
Completeness [%]99.298.7
Redundancy2.9
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1EVAPORATION6.5277.1512%MPD 0.1M MES-KOH pH6.5 50mM KCL 10mM NH4-CL 15mM Mg2CL, EVAPORATION, temperature 277.15K

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