4I7H
Structural basis for peroxide sensing and gene regulation by PerR from Streptococcus pyogenes
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.3.1 |
Synchrotron site | ALS |
Beamline | 8.3.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-08-30 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.9796, 0.957, 0.9798 |
Spacegroup name | P 63 2 2 |
Unit cell lengths | 118.910, 118.910, 118.611 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 47.231 - 2.000 |
R-factor | 0.2312 |
Rwork | 0.229 |
R-free | 0.26450 |
Structure solution method | MAD |
RMSD bond length | 0.009 |
RMSD bond angle | 1.125 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | SOLVE |
Refinement software | PHENIX (1.7.2_869) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 2.000 |
Number of reflections | 33859 |
Completeness [%] | 99.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 298 | 2 M sodium chloride, 0.1 M Tris-HCl, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 298 | 2 M sodium chloride, 0.1 M Tris-HCl, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |