3RNU
Structural Basis of Cytosolic DNA Sensing by Innate Immune Receptors
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-B |
| Synchrotron site | APS |
| Beamline | 23-ID-B |
| Temperature [K] | 298 |
| Detector technology | CCD |
| Collection date | 2010-07-08 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.0332 |
| Spacegroup name | H 3 |
| Unit cell lengths | 126.841, 126.841, 163.868 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 41.388 - 2.502 |
| R-factor | 0.1782 |
| Rwork | 0.176 |
| R-free | 0.21590 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3rlo |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.932 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: dev_736)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.540 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.078 | 0.505 |
| Number of reflections | 33125 | |
| <I/σ(I)> | 21.68 | 2.57 |
| Completeness [%] | 97.6 | 84.1 |
| Redundancy | 5.6 | 4.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | 20% PEG3350, 0.1 M K Formate, 0.1 M HEPES, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
