3RN2
Structural Basis of Cytosolic DNA Recognition by Innate Immune Receptors
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-B |
Synchrotron site | APS |
Beamline | 23-ID-B |
Temperature [K] | 298 |
Detector technology | CCD |
Collection date | 2010-10-13 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 1.0332 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 58.560, 74.770, 65.520 |
Unit cell angles | 90.00, 89.73, 90.00 |
Refinement procedure
Resolution | 43.765 - 2.550 |
R-factor | 0.2103 |
Rwork | 0.208 |
R-free | 0.25250 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | code 3RLO |
RMSD bond length | 0.005 |
RMSD bond angle | 0.544 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: dev_723)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.590 |
High resolution limit [Å] | 2.550 | 2.550 |
Rmerge | 0.050 | 0.617 |
Number of reflections | 18538 | |
<I/σ(I)> | 25.49 | 3.11 |
Completeness [%] | 99.8 | 99.8 |
Redundancy | 4.16 | 4.25 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | 8% PEG1000, 0.1 M KCl, 10 mM MgCl2, 0.1 M MES, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |