3OV1
Crystal Structure of the Grb2 SH2 Domain in Complex with a pYXN-Derived Tripeptide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU200 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2010-04-10 |
Detector | RIGAKU RAXIS IV++ |
Wavelength(s) | 1.5418 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 42.131, 42.131, 108.983 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 23.040 - 1.600 |
R-factor | 0.16381 |
Rwork | 0.161 |
R-free | 0.21383 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3c71 |
RMSD bond length | 0.030 |
RMSD bond angle | 2.553 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.660 |
High resolution limit [Å] | 1.600 | 1.600 |
Number of reflections | 12936 | |
<I/σ(I)> | 22.3 | 17.5 |
Completeness [%] | 94.0 | 88.3 |
Redundancy | 13.7 | 13.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | 0.1 M HEPES, 20% w/v Polyethylene Glycol 10,000, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |