3LFV
crystal structure of unliganded PDE5A GAF domain
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X29A |
Synchrotron site | NSLS |
Beamline | X29A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-06-03 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.9791, 0.9796, 0.94, 1.0 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 144.189, 144.189, 134.867 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 30.000 - 2.800 |
R-factor | 0.24267 |
Rwork | 0.240 |
R-free | 0.28456 |
Structure solution method | MAD |
Starting model (for MR) | None |
RMSD bond length | 0.005 |
RMSD bond angle | 0.667 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SOLVE |
Refinement software | REFMAC (5.5.0072) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.900 |
High resolution limit [Å] | 2.800 | 2.800 |
Rmerge | 0.060 | 0.840 |
Number of reflections | 40247 | |
<I/σ(I)> | 19.5 | 4.7 |
Completeness [%] | 99.8 | 99.3 |
Redundancy | 21.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 277 | The unliganded PDE5A (20 mg/mL) was crystallized by vapor diffusion against the well buffer of 10% PEG3350, 5% MPD, 0.1 M sodium citrate, pH5.6, 20 mM DTT, VAPOR DIFFUSION, HANGING DROP, temperature 277K |