2Z5Y
Crystal Structure of Human Monoamine Oxidase A (G110A) with Harmine
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL44XU |
Synchrotron site | SPring-8 |
Beamline | BL44XU |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-07-12 |
Detector | Bruker DIP-6040 |
Wavelength(s) | 0.9 |
Spacegroup name | C 2 2 2 |
Unit cell lengths | 135.547, 217.364, 54.804 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 44.240 - 2.170 |
R-factor | 0.19592 |
Rwork | 0.193 |
R-free | 0.24358 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1o5w |
RMSD bond length | 0.019 |
RMSD bond angle | 1.812 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | MOLREP |
Refinement software | REFMAC (5.3.0021) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.570 | 2.250 |
High resolution limit [Å] | 2.160 | 2.160 |
Rmerge | 0.079 | 0.310 |
Number of reflections | 31614 | |
<I/σ(I)> | 7.1 | 1.5 |
Completeness [%] | 71.4 | 21.5 |
Redundancy | 2.8 | 1.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5 | 277 | 1.6M AMMONIUM SULFATE, 100mM CITRIC ACID, pH 5.00, VAPOR DIFFUSION, SITTING DROP, temperature 277K |