2WD0

CRYSTAL STRUCTURE OF NONSYNDROMIC DEAFNESS (DFNB12) ASSOCIATED MUTANT D124G OF MOUSE CADHERIN-23 EC1-2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X25
Synchrotron site	NSLS
Beamline	X25
Temperature [K]	100
Detector technology	CCD
Collection date	2009-01-28
Detector	ADSC CCD
Spacegroup name	P 42 21 2
Unit cell lengths	179.693, 179.693, 63.982
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.770 - 2.740
R-factor	0.22089
Rwork	0.219
R-free	0.24865
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2wcp
RMSD bond length	0.011
RMSD bond angle	1.099
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.5.0070)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.800
High resolution limit [Å]	2.750	2.750
Rmerge	0.090	0.490
Number of reflections	28267
<I/σ(I)>	13.8	4.47
Completeness [%]	97.9	97.4
Redundancy	7.4	5.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		7.1		0.1M SODIUM CACODYLATE PH 7.1, 1M NACL