1IKX
K103N Mutant HIV-1 Reverse Transcriptase in Complex with the Inhibitor PNU142721
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX II BEAMLINE I711 |
| Synchrotron site | MAX II |
| Beamline | I711 |
| Temperature [K] | 270 |
| Detector technology | IMAGE PLATE |
| Collection date | 2000-02-12 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.0159 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 119.900, 156.400, 156.900 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 23.630 - 2.800 |
| R-factor | 0.21 * |
| Rwork | 0.210 |
| R-free | 0.27400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.300 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 23.630 | 23.630 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.075 | 0.374 |
| Total number of observations | 289120 * | |
| Number of reflections | 32921 | 3306 * |
| <I/σ(I)> | 12.2 | |
| Completeness [%] | 81.3 | 92.3 * |
| Redundancy | 90.2 | 4.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 7.2 | 295 | Ammonium sulphate, pH 7.2, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 20 (mg/ml) | |
| 2 | 1 | drop | DMSO | 30 (mM) | |
| 3 | 1 | reservoir | ammonium sulfate | 1.4 (M) | |
| 4 | 1 | reservoir | HEPES | 50 (mM) | pH7.2 |
| 5 | 1 | reservoir | 5 (mM) | ||
| 6 | 1 | reservoir | 300 (mM) |
