1GYQ
CRYSTAL STRUCTURE OF GLYCOSOMAL GLYCERALDEHYDE FROM LEISHMANIA MEXICANA IN COMPLEX WITH N6-BENZYL-NAD
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL7-1 |
Synchrotron site | SSRL |
Beamline | BL7-1 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 1997-12-01 |
Detector | MARRESEARCH |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 97.330, 125.810, 137.790 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | ? - 3.400 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1gyp |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | RAVE |
Refinement software | X-PLOR |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 8.000 | 3.520 |
High resolution limit [Å] | 3.400 | 3.400 |
Rmerge | 0.138 | 0.433 |
Number of reflections | 39118 | |
<I/σ(I)> | 5.9 | 1.8 |
Completeness [%] | 84.0 | 83 |
Redundancy | 2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 * | 2UL OF SOLUTION CONTAINING 2.25 MG L. MEXICANA GAPDH, 1MM DDT, 1MM EDTA, 1MMPMSF 0.4 MM N6-BENZYL-NAD IN 10MM TRIETHANOLAMINE BUFFER PH 7.0 WAS MIXED WITH 1UL OF RESERVOIR SOLUTION CONTAINING 25 %PEG5000MME, 100MM TRIS PH8.0 AND EQUILIBRATED IN SITTING DROP TRAYS, pH 7.6, VAPOR DIFFUSION, SITTING DROP |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 2.25 (mg/ml) | |
2 | 1 | drop | dithiothreitol | 1 (mM) | |
3 | 1 | drop | EDTA | 1 (mM) | |
4 | 1 | drop | phenylmethylsulfonyl fluoride | 1 (mM) | |
5 | 1 | drop | N6-benzyl-NAD+ | 0.4 (mM) | |
6 | 1 | drop | triethanolamine | 10 (mM) | |
7 | 1 | reservoir | mPEG5000 | 25 (%) | |
8 | 1 | reservoir | Tris-HCl | 100 (mM) |