Green Fluorescent Protein / Green fluorescent protein / Green fluorescent protein, GFP / Green fluorescent protein-related / Green fluorescent protein / Green fluorescent protein / Beta Barrel / Mainly Beta Similarity search - Domain/homology
SHEET DETERMINATION METHOD: DSSP THE SHEETS PRESENTED AS "AA" IN EACH CHAIN ON SHEET RECORDS BELOW ... SHEET DETERMINATION METHOD: DSSP THE SHEETS PRESENTED AS "AA" IN EACH CHAIN ON SHEET RECORDS BELOW IS ACTUALLY AN 11-STRANDED BARREL THIS IS REPRESENTED BY A 12-STRANDED SHEET IN WHICH THE FIRST AND LAST STRANDS ARE IDENTICAL.
Mass: 18.015 Da / Num. of mol.: 143 / Source method: isolated from a natural source / Formula: H2O
Compound details
ENGINEERED RESIDUE IN CHAIN A, PHE 64 TO LEU ENGINEERED RESIDUE IN CHAIN A, TYR 66 TO TRP ...ENGINEERED RESIDUE IN CHAIN A, PHE 64 TO LEU ENGINEERED RESIDUE IN CHAIN A, TYR 66 TO TRP ENGINEERED RESIDUE IN CHAIN A, SER 72 TO ALA ENGINEERED RESIDUE IN CHAIN A, ASN 146 TO ILE ENGINEERED RESIDUE IN CHAIN A, HIS 148 TO GLY ENGINEERED RESIDUE IN CHAIN A, MET 153 TO THR ENGINEERED RESIDUE IN CHAIN A, VAL 163 TO ALA ENGINEERED RESIDUE IN CHAIN A, SER 175 TO GLY ENGINEERED RESIDUE IN CHAIN A, VAL 224 TO LEU ENGINEERED RESIDUE IN CHAIN A, HIS 231 TO LEU
Sequence details
CHROMOPHORE RESULTING FROM THE AUTOCATALYTIC CYCLISATION OF RESIDUES 65 TO 67.
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Experimental details
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Experiment
Experiment
Method: X-RAY DIFFRACTION / Number of used crystals: 1
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Sample preparation
Crystal
Density Matthews: 2 Å3/Da / Density % sol: 38 % / Description: NONE
Crystal grow
pH: 7 Details: 35 MG/ML PROTEIN, 14% PEG8000, 100 MM MGCL2, 100 MM HEPES PH 7.00
Resolution: 1.63→41.26 Å / Cor.coef. Fo:Fc: 0.97 / Cor.coef. Fo:Fc free: 0.952 / SU B: 3.74 / SU ML: 0.057 / Cross valid method: THROUGHOUT / ESU R: 0.122 / ESU R Free: 0.09 / Stereochemistry target values: MAXIMUM LIKELIHOOD Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS. U VALUES REFINED INDIVIDUALLY
Rfactor
Num. reflection
% reflection
Selection details
Rfree
0.19389
1358
5 %
RANDOM
Rwork
0.1458
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obs
0.14819
25710
96.45 %
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Solvent computation
Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å / Solvent model: MASK