7DQ6
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![BU of 7dq6 by Molmil](/molmil-images/mine/7dq6) | Crystal structure of HitB in complex with (S)-beta-3-Br-phenylalanine sulfamoyladenosine | Descriptor: | CALCIUM ION, Putative ATP-dependent b-aminoacyl-ACP synthetase, [(2R,3S,4R,5R)-5-(6-aminopurin-9-yl)-3,4-bis(oxidanyl)oxolan-2-yl]methyl N-[(3S)-3-azanyl-3-(3-bromophenyl)propanoyl]sulfamate | Authors: | Kudo, F, Takahashi, S, Miyanaga, A, Nakazawa, Y, Eguchi, T. | Deposit date: | 2020-12-22 | Release date: | 2021-03-03 | Last modified: | 2023-11-29 | Method: | X-RAY DIFFRACTION (2.6 Å) | Cite: | Mutational Biosynthesis of Hitachimycin Analogs Controlled by the beta-Amino Acid-Selective Adenylation Enzyme HitB. Acs Chem.Biol., 16, 2021
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7DQ5
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![BU of 7dq5 by Molmil](/molmil-images/mine/7dq5) | Crystal structure of HitB in complex with (S)-beta-phenylalanine sulfamoyladenosine | Descriptor: | CALCIUM ION, Putative ATP-dependent b-aminoacyl-ACP synthetase, [(2~{R},3~{S},4~{R},5~{R})-5-(6-aminopurin-9-yl)-3,4-bis(oxidanyl)oxolan-2-yl]methyl ~{N}-[(3~{S})-3-azanyl-3-phenyl-propanoyl]sulfamate | Authors: | Kudo, F, Takahashi, S, Miyanaga, A, Nakazawa, Y, Eguchi, T. | Deposit date: | 2020-12-22 | Release date: | 2021-03-03 | Last modified: | 2023-11-29 | Method: | X-RAY DIFFRACTION (2.45 Å) | Cite: | Mutational Biosynthesis of Hitachimycin Analogs Controlled by the beta-Amino Acid-Selective Adenylation Enzyme HitB. Acs Chem.Biol., 16, 2021
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6JW8
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![BU of 6jw8 by Molmil](/molmil-images/mine/6jw8) | The crystal structure of KanD2 in complex with NADH and 3"-deamino-3"-hydroxykanamycin B | Descriptor: | (2S,3R,4S,5S,6R)-2-[(1S,2S,3R,4S,6R)-3-[(2R,3R,4R,5S,6R)-6-(aminomethyl)-3-azanyl-4,5-bis(oxidanyl)oxan-2-yl]oxy-4,6-bis(azanyl)-2-oxidanyl-cyclohexyl]oxy-6-(hydroxymethyl)oxane-3,4,5-triol, 1,4-DIHYDRONICOTINAMIDE ADENINE DINUCLEOTIDE, Dehydrogenase | Authors: | Kudo, F, Kitayama, Y, Miyanaga, A, Hirayama, A, Eguchi, T. | Deposit date: | 2019-04-18 | Release date: | 2020-04-15 | Last modified: | 2023-11-22 | Method: | X-RAY DIFFRACTION (2.4 Å) | Cite: | Biochemical and structural analysis of a dehydrogenase, KanD2, and an aminotransferase, KanS2, that are responsible for the construction of the kanosamine moiety in kanamycin biosynthesis. Biochemistry, 59, 2020
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6JW7
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![BU of 6jw7 by Molmil](/molmil-images/mine/6jw7) | The crystal structure of KanD2 in complex with NADH and 3"-deamino-3"-hydroxykanamycin A | Descriptor: | (2R,3S,4S,5R,6R)-2-(aminomethyl)-6-[(1R,2S,3S,4R,6S)-4,6-bis(azanyl)-3-[(2S,3R,4S,5S,6R)-6-(hydroxymethyl)-3,4,5-tris(oxidanyl)oxan-2-yl]oxy-2-oxidanyl-cyclohexyl]oxy-oxane-3,4,5-triol, 1,4-DIHYDRONICOTINAMIDE ADENINE DINUCLEOTIDE, Dehydrogenase | Authors: | Kudo, F, Kitayama, Y, Miyanaga, A, Hirayama, A, Eguchi, T. | Deposit date: | 2019-04-18 | Release date: | 2020-04-15 | Last modified: | 2023-11-22 | Method: | X-RAY DIFFRACTION (2.36 Å) | Cite: | Biochemical and structural analysis of a dehydrogenase, KanD2, and an aminotransferase, KanS2, that are responsible for the construction of the kanosamine moiety in kanamycin biosynthesis. Biochemistry, 59, 2020
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6JW6
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![BU of 6jw6 by Molmil](/molmil-images/mine/6jw6) | The crystal structure of KanD2 in complex with NAD | Descriptor: | Dehydrogenase, NICOTINAMIDE-ADENINE-DINUCLEOTIDE | Authors: | Kudo, F, Kitayama, Y, Miyanaga, A, Hirayama, A, Eguchi, T. | Deposit date: | 2019-04-18 | Release date: | 2020-04-15 | Last modified: | 2023-11-22 | Method: | X-RAY DIFFRACTION (2.8 Å) | Cite: | Biochemical and structural analysis of a dehydrogenase, KanD2, and an aminotransferase, KanS2, that are responsible for the construction of the kanosamine moiety in kanamycin biosynthesis. Biochemistry, 59, 2020
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8IN9
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![BU of 8in9 by Molmil](/molmil-images/mine/8in9) | The structure of the GfsA KSQ-AT didomain in complex with the GfsA ACP domain | Descriptor: | N-[2-(acetylamino)ethyl]-N~3~-[(2R)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alaninamide, Polyketide synthase | Authors: | Chisuga, T, Murakami, S, Miyanaga, A, Kudo, F, Eguchi, T. | Deposit date: | 2023-03-09 | Release date: | 2023-05-31 | Last modified: | 2023-06-28 | Method: | X-RAY DIFFRACTION (3.4 Å) | Cite: | Structure-Based Analysis of Transient Interactions between Ketosynthase-like Decarboxylase and Acyl Carrier Protein in a Loading Module of Modular Polyketide Synthase. Acs Chem.Biol., 18, 2023
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6M01
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![BU of 6m01 by Molmil](/molmil-images/mine/6m01) | The structure of HitB-HitD complex | Descriptor: | ADENOSINE-5'-DIPHOSPHATE, GLYCEROL, N-[2-(acetylamino)ethyl]-N~3~-[(2R)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alaninamide, ... | Authors: | Miyanaga, A, Kurihara, S, Kudo, F, Eguchi, T. | Deposit date: | 2020-02-19 | Release date: | 2020-07-08 | Last modified: | 2023-11-29 | Method: | X-RAY DIFFRACTION (2 Å) | Cite: | Structural Characterization of Complex of Adenylation Domain and Carrier Protein by Using Pantetheine Cross-Linking Probe. Acs Chem.Biol., 15, 2020
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6AKD
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![BU of 6akd by Molmil](/molmil-images/mine/6akd) | Crystal structure of IdnL7 | Descriptor: | '5'-O-(N-(L-ALANYL)-SULFAMOYL)ADENOSINE, AMP-dependent synthetase and ligase, GLYCEROL | Authors: | Cieslak, J, Miyanaga, A, Kudo, F, Eguchi, T. | Deposit date: | 2018-08-31 | Release date: | 2019-03-06 | Last modified: | 2023-11-22 | Method: | X-RAY DIFFRACTION (2.1 Å) | Cite: | Functional and structural characterization of IdnL7, an adenylation enzyme involved in incednine biosynthesis. Acta Crystallogr F Struct Biol Commun, 75, 2019
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8H6S
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![BU of 8h6s by Molmil](/molmil-images/mine/8h6s) | Structure of acyltransferase VinK in complex with the loading acyl carrier protein of vicenistatin PKS | Descriptor: | MAGNESIUM ION, Malonyl-CoA-[acyl-carrier-protein] transacylase, N-[2-(acetylamino)ethyl]-N~3~-[(2R)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alaninamide, ... | Authors: | Kawada, K, Miyanaga, A, Chisuga, T, Kudo, F, Eguchi, T. | Deposit date: | 2022-10-18 | Release date: | 2022-12-21 | Last modified: | 2023-11-29 | Method: | X-RAY DIFFRACTION (3 Å) | Cite: | Structural Basis of Transient Interactions of Acyltransferase VinK with the Loading Acyl Carrier Protein of the Vicenistatin Modular Polyketide Synthase. Biochemistry, 62, 2023
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8YYQ
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![BU of 8yyq by Molmil](/molmil-images/mine/8yyq) | Structure of the HitB F328L mutant | Descriptor: | Putative ATP-dependent b-aminoacyl-ACP synthetase, [(2~{R},3~{S},4~{R},5~{R})-5-(6-aminopurin-9-yl)-3,4-bis(oxidanyl)oxolan-2-yl]methyl ~{N}-[(3~{S})-3-azanyl-3-(3-cyanophenyl)propanoyl]sulfamate | Authors: | Wang, D, Miyanaga, A, Chisuga, T, Kudo, F, Eguchi, T. | Deposit date: | 2024-04-04 | Release date: | 2024-06-05 | Method: | X-RAY DIFFRACTION (1.95 Å) | Cite: | Engineering the Substrate Specificity of (S)-beta-Phenylalanine Adenylation Enzyme HitB. Chembiochem, 2024
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8YYR
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![BU of 8yyr by Molmil](/molmil-images/mine/8yyr) | Structure of the HitB T293G mutant | Descriptor: | Putative ATP-dependent b-aminoacyl-ACP synthetase, [(2~{R},3~{S},4~{R},5~{R})-5-(6-aminopurin-9-yl)-3,4-bis(oxidanyl)oxolan-2-yl]methyl ~{N}-[(3~{S})-3-azanyl-3-(2-bromophenyl)propanoyl]sulfamate | Authors: | Wang, D, Miyanaga, A, Chisuga, T, Kudo, F, Eguchi, T. | Deposit date: | 2024-04-04 | Release date: | 2024-06-05 | Method: | X-RAY DIFFRACTION (1.8 Å) | Cite: | Engineering the Substrate Specificity of (S)-beta-Phenylalanine Adenylation Enzyme HitB. Chembiochem, 2024
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5CZD
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![BU of 5czd by Molmil](/molmil-images/mine/5czd) | The complex structure of VinK with VinL | Descriptor: | 1,1'-ethane-1,2-diyldipyrrolidine-2,5-dione, 4'-PHOSPHOPANTETHEINE, Acyl-carrier-protein, ... | Authors: | Miyanaga, A, Iwasawa, S, Shinohara, Y, Kudo, F, Eguchi, T. | Deposit date: | 2015-07-31 | Release date: | 2016-02-03 | Last modified: | 2023-11-08 | Method: | X-RAY DIFFRACTION (2.34 Å) | Cite: | Structure-based analysis of the molecular interactions between acyltransferase and acyl carrier protein in vicenistatin biosynthesis. Proc.Natl.Acad.Sci.USA, 113, 2016
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5CZC
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![BU of 5czc by Molmil](/molmil-images/mine/5czc) | The structure of VinK | Descriptor: | CALCIUM ION, GLYCEROL, Malonyl-CoA-[acyl-carrier-protein] transacylase | Authors: | Miyanaga, A, Iwasawa, S, Shinohara, Y, Kudo, F, Eguchi, T. | Deposit date: | 2015-07-31 | Release date: | 2016-02-03 | Last modified: | 2024-03-20 | Method: | X-RAY DIFFRACTION (1.8 Å) | Cite: | Structure-based analysis of the molecular interactions between acyltransferase and acyl carrier protein in vicenistatin biosynthesis. Proc.Natl.Acad.Sci.USA, 113, 2016
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7VEE
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![BU of 7vee by Molmil](/molmil-images/mine/7vee) | The ligand-free structure of GfsA KSQ-AT didomain | Descriptor: | GLYCEROL, Polyketide synthase | Authors: | Chisuga, T, Miyanaga, A, Nagai, A, Kudo, F, Eguchi, T. | Deposit date: | 2021-09-08 | Release date: | 2022-01-12 | Last modified: | 2023-11-29 | Method: | X-RAY DIFFRACTION (2.55 Å) | Cite: | Structural Insight into the Reaction Mechanism of Ketosynthase-Like Decarboxylase in a Loading Module of Modular Polyketide Synthases. Acs Chem.Biol., 17, 2022
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7VEF
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![BU of 7vef by Molmil](/molmil-images/mine/7vef) | The structure of GfsA KSQ-AT didomain in complex with a malonate substrate analog | Descriptor: | GLYCEROL, N-(2-acetamidoethyl)-2-nitro-ethanamide, Polyketide synthase | Authors: | Chisuga, T, Miyanaga, A, Nagai, A, Kudo, F, Eguchi, T. | Deposit date: | 2021-09-08 | Release date: | 2022-01-12 | Last modified: | 2023-11-29 | Method: | X-RAY DIFFRACTION (2.65 Å) | Cite: | Structural Insight into the Reaction Mechanism of Ketosynthase-Like Decarboxylase in a Loading Module of Modular Polyketide Synthases. Acs Chem.Biol., 17, 2022
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7F2R
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![BU of 7f2r by Molmil](/molmil-images/mine/7f2r) | Crystal structure of VinK-VinL covalent complex formed with a pantetheineamide cross-linking probe | Descriptor: | Acyl-carrier-protein, Malonyl-CoA-[acyl-carrier-protein] transacylase, N-[2-(acetylamino)ethyl]-N~3~-[(2R)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alaninamide | Authors: | Miyanaga, A, Ouchi, R, Kudo, F, Eguchi, T. | Deposit date: | 2021-06-14 | Release date: | 2021-09-01 | Last modified: | 2023-11-29 | Method: | X-RAY DIFFRACTION (1.95 Å) | Cite: | Complex structure of the acyltransferase VinK and the carrier protein VinL with a pantetheine cross-linking probe. Acta Crystallogr.,Sect.F, 77, 2021
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5JJP
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![BU of 5jjp by Molmil](/molmil-images/mine/5jjp) | Crystal structure of CmiS6 | Descriptor: | Nonribosomal peptide synthase | Authors: | Cieslak, J, Miyanaga, A, Kudo, F, Eguchi, T. | Deposit date: | 2016-04-25 | Release date: | 2017-03-22 | Last modified: | 2023-11-08 | Method: | X-RAY DIFFRACTION (2.3 Å) | Cite: | Biochemical characterization and structural insight into aliphatic beta-amino acid adenylation enzymes IdnL1 and CmiS6 Proteins, 85, 2017
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5JJQ
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![BU of 5jjq by Molmil](/molmil-images/mine/5jjq) | Crystal structure of IdnL1 | Descriptor: | 5'-O-[(R)-{[(3S)-3-aminobutanoyl]oxy}(hydroxy)phosphoryl]adenosine, AMP-dependent synthetase and ligase, CHLORIDE ION | Authors: | Cieslak, J, Miyanaga, A, Kudo, F, Eguchi, T. | Deposit date: | 2016-04-25 | Release date: | 2017-03-22 | Last modified: | 2023-11-08 | Method: | X-RAY DIFFRACTION (2.6 Å) | Cite: | Biochemical characterization and structural insight into aliphatic beta-amino acid adenylation enzymes IdnL1 and CmiS6 Proteins, 85, 2017
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8K4R
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![BU of 8k4r by Molmil](/molmil-images/mine/8k4r) | Structure of VinM-VinL complex | Descriptor: | Acyl-carrier-protein, Non-ribosomal peptide synthetase, SODIUM ION, ... | Authors: | Miyanaga, A, Nagata, K, Nakajima, J, Chisuga, T, Kudo, F, Eguchi, T. | Deposit date: | 2023-07-20 | Release date: | 2023-11-01 | Last modified: | 2023-11-29 | Method: | X-RAY DIFFRACTION (2.3 Å) | Cite: | Structural Basis of Amide-Forming Adenylation Enzyme VinM in Vicenistatin Biosynthesis. Acs Chem.Biol., 18, 2023
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7CL5
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![BU of 7cl5 by Molmil](/molmil-images/mine/7cl5) | The crystal structure of KanJ in complex with kanamycin B and N-oxalylglycine | Descriptor: | (1R,2S,3S,4R,6S)-4,6-DIAMINO-3-[(3-AMINO-3-DEOXY-ALPHA-D-GLUCOPYRANOSYL)OXY]-2-HYDROXYCYCLOHEXYL 2,6-DIAMINO-2,6-DIDEOXY-ALPHA-D-GLUCOPYRANOSIDE, Kanamycin B dioxygenase, N-OXALYLGLYCINE, ... | Authors: | Kitayama, Y, Miyanaga, A, Kudo, F, Eguchi, T. | Deposit date: | 2020-07-20 | Release date: | 2021-01-13 | Last modified: | 2023-11-29 | Method: | X-RAY DIFFRACTION (2.5 Å) | Cite: | Stepwise Post-glycosylation Modification of Sugar Moieties in Kanamycin Biosynthesis. Chembiochem, 22, 2021
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7CL4
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![BU of 7cl4 by Molmil](/molmil-images/mine/7cl4) | The crystal structure of KanJ in complex with N-oxalylglycine | Descriptor: | Kanamycin B dioxygenase, N-OXALYLGLYCINE, NICKEL (II) ION | Authors: | Kitayama, Y, Miyanaga, A, Kudo, F, Eguchi, T. | Deposit date: | 2020-07-20 | Release date: | 2021-01-13 | Last modified: | 2023-11-29 | Method: | X-RAY DIFFRACTION (2.25 Å) | Cite: | Stepwise Post-glycosylation Modification of Sugar Moieties in Kanamycin Biosynthesis. Chembiochem, 22, 2021
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7CL6
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![BU of 7cl6 by Molmil](/molmil-images/mine/7cl6) | The crystal structure of KanJ in complex with neamine and N-oxalylglycine | Descriptor: | (1R,2R,3S,4R,6S)-4,6-diamino-2,3-dihydroxycyclohexyl 2,6-diamino-2,6-dideoxy-alpha-D-glucopyranoside, Kanamycin B dioxygenase, N-OXALYLGLYCINE, ... | Authors: | Kitayama, Y, Miyanaga, A, Kudo, F, Eguchi, T. | Deposit date: | 2020-07-20 | Release date: | 2021-01-13 | Last modified: | 2023-11-29 | Method: | X-RAY DIFFRACTION (2.44 Å) | Cite: | Stepwise Post-glycosylation Modification of Sugar Moieties in Kanamycin Biosynthesis. Chembiochem, 22, 2021
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7CL2
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![BU of 7cl2 by Molmil](/molmil-images/mine/7cl2) | The crystal structure of KanJ | Descriptor: | GLYCEROL, Kanamycin B dioxygenase, NICKEL (II) ION, ... | Authors: | Kitayama, Y, Miyanaga, A, Kudo, F, Eguchi, T. | Deposit date: | 2020-07-20 | Release date: | 2021-01-13 | Last modified: | 2024-03-27 | Method: | X-RAY DIFFRACTION (2 Å) | Cite: | Stepwise Post-glycosylation Modification of Sugar Moieties in Kanamycin Biosynthesis. Chembiochem, 22, 2021
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7CL3
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![BU of 7cl3 by Molmil](/molmil-images/mine/7cl3) | The crystal structure of KanJ in complex with kanamycin B | Descriptor: | (1R,2S,3S,4R,6S)-4,6-DIAMINO-3-[(3-AMINO-3-DEOXY-ALPHA-D-GLUCOPYRANOSYL)OXY]-2-HYDROXYCYCLOHEXYL 2,6-DIAMINO-2,6-DIDEOXY-ALPHA-D-GLUCOPYRANOSIDE, Kanamycin B dioxygenase, NICKEL (II) ION, ... | Authors: | Kitayama, Y, Miyanaga, A, Kudo, F, Eguchi, T. | Deposit date: | 2020-07-20 | Release date: | 2021-01-13 | Last modified: | 2023-11-29 | Method: | X-RAY DIFFRACTION (2.2 Å) | Cite: | Stepwise Post-glycosylation Modification of Sugar Moieties in Kanamycin Biosynthesis. Chembiochem, 22, 2021
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5ZK4
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![BU of 5zk4 by Molmil](/molmil-images/mine/5zk4) | The structure of DSZS acyltransferase with carrier protein | Descriptor: | DisA protein, DisD protein, N-[2-(acetylamino)ethyl]-N~3~-[(2R)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alaninamide, ... | Authors: | Miyanaga, A, Ouchi, R, Kudo, F, Eguchi, T. | Deposit date: | 2018-03-23 | Release date: | 2018-06-13 | Last modified: | 2023-11-22 | Method: | X-RAY DIFFRACTION (2.03 Å) | Cite: | Structural basis of protein-protein interactions between a trans-acting acyltransferase and acyl carrier protein in polyketide disorazole biosynthesis J. Am. Chem. Soc., 140, 2018
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