7Z4H
| SpCas9 bound to 14-nucleotide complementary DNA substrate | Descriptor: | CRISPR-associated endonuclease Cas9/Csn1, Non-target strand of 14-nucleotide complementary DNA substrate, Target strand of 14-nucleotide complementary DNA substrate, ... | Authors: | Pacesa, M, Jinek, M. | Deposit date: | 2022-03-03 | Release date: | 2022-08-31 | Last modified: | 2022-09-14 | Method: | ELECTRON MICROSCOPY (3.49 Å) | Cite: | R-loop formation and conformational activation mechanisms of Cas9. Nature, 609, 2022
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7Z4J
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7ZO1
| SpCas9 bound to CD34 off-target9 DNA substrate | Descriptor: | CD34 off-target9 DNA non-target strand, CD34 off-target9 DNA target strand, CD34 sgRNA, ... | Authors: | Pacesa, M, Jinek, M. | Deposit date: | 2022-04-23 | Release date: | 2022-10-26 | Last modified: | 2024-01-31 | Method: | X-RAY DIFFRACTION (2.4 Å) | Cite: | Structural basis for Cas9 off-target activity. Cell, 185, 2022
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7QQS
| SpCas9 bound to FANCF on-target DNA substrate | Descriptor: | CRISPR-associated endonuclease Cas9/Csn1, FANCF on-target non-target strand, FANCF on-target target strand, ... | Authors: | Pacesa, M, Jinek, M. | Deposit date: | 2022-01-10 | Release date: | 2022-10-26 | Last modified: | 2024-01-31 | Method: | X-RAY DIFFRACTION (2.4 Å) | Cite: | Structural basis for Cas9 off-target activity. Cell, 185, 2022
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7QR8
| SpCas9 bound to PTPRC off-target1 DNA substrate | Descriptor: | CRISPR-associated endonuclease Cas9/Csn1, MAGNESIUM ION, POTASSIUM ION, ... | Authors: | Pacesa, M, Jinek, M. | Deposit date: | 2022-01-10 | Release date: | 2022-10-26 | Last modified: | 2024-01-31 | Method: | X-RAY DIFFRACTION (2.75 Å) | Cite: | Structural basis for Cas9 off-target activity. Cell, 185, 2022
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7QQQ
| SpCas9 bound to AAVS1 off-target4 DNA substrate | Descriptor: | 1,2-ETHANEDIOL, AAVS1 off-target4 non-target strand, AAVS1 off-target4 target strand, ... | Authors: | Pacesa, M, Jinek, M. | Deposit date: | 2022-01-10 | Release date: | 2022-10-26 | Last modified: | 2024-01-31 | Method: | X-RAY DIFFRACTION (2.65 Å) | Cite: | Structural basis for Cas9 off-target activity. Cell, 185, 2022
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7QQO
| SpCas9 bound to AAVS1 off-target1 DNA substrate | Descriptor: | AAVS1 off-target1 non-target strand, AAVS1 off-target1 target strand, AAVS1 sgRNA, ... | Authors: | Pacesa, M, Jinek, M. | Deposit date: | 2022-01-10 | Release date: | 2022-10-26 | Last modified: | 2024-01-31 | Method: | X-RAY DIFFRACTION (3 Å) | Cite: | Structural basis for Cas9 off-target activity. Cell, 185, 2022
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7QQV
| SpCas9 bound to FANCF off-target3 DNA substrate | Descriptor: | CRISPR-associated endonuclease Cas9/Csn1, FANCF off-target3 non-target strand, FANCF off-target3 target strand, ... | Authors: | Pacesa, M, Jinek, M. | Deposit date: | 2022-01-10 | Release date: | 2022-10-26 | Last modified: | 2024-01-31 | Method: | X-RAY DIFFRACTION (2.25 Å) | Cite: | Structural basis for Cas9 off-target activity. Cell, 185, 2022
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7QQW
| SpCas9 bound to FANCF off-target4 DNA substrate | Descriptor: | CRISPR-associated endonuclease Cas9/Csn1, FANCF off-target4 non-target strand, FANCF off-target4 target strand, ... | Authors: | Pacesa, M, Jinek, M. | Deposit date: | 2022-01-10 | Release date: | 2022-10-26 | Last modified: | 2024-01-31 | Method: | X-RAY DIFFRACTION (3.1 Å) | Cite: | Structural basis for Cas9 off-target activity. Cell, 185, 2022
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7QR5
| SpCas9 bound to FANCF off-target6 DNA substrate | Descriptor: | 1,2-ETHANEDIOL, CRISPR-associated endonuclease Cas9/Csn1, FANCF off-target6 non-target strand, ... | Authors: | Pacesa, M, Jinek, M. | Deposit date: | 2022-01-17 | Release date: | 2022-10-26 | Last modified: | 2024-01-31 | Method: | X-RAY DIFFRACTION (3.3 Å) | Cite: | Structural basis for Cas9 off-target activity. Cell, 185, 2022
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7QQP
| SpCas9 bound to AAVS1 off-target3 DNA substrate | Descriptor: | 1,2-ETHANEDIOL, AAVS1 off-target3 non-target strand, AAVS1 off-target3 target strand, ... | Authors: | Pacesa, M, JInek, M. | Deposit date: | 2022-01-10 | Release date: | 2022-10-26 | Last modified: | 2024-01-31 | Method: | X-RAY DIFFRACTION (2.6 Å) | Cite: | Structural basis for Cas9 off-target activity. Cell, 185, 2022
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7QQR
| SpCas9 bound to AAVS1 off-target5 DNA substrate | Descriptor: | 1,2-ETHANEDIOL, AAVS1 off-target5 non-target strand, AAVS1 off-target5 target strand, ... | Authors: | Pacesa, M, Jinek, M. | Deposit date: | 2022-01-10 | Release date: | 2022-10-26 | Last modified: | 2024-01-31 | Method: | X-RAY DIFFRACTION (2.75 Å) | Cite: | Structural basis for Cas9 off-target activity. Cell, 185, 2022
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7QQX
| SpCas9 bound to FANCF off-target5 DNA substrate | Descriptor: | CRISPR-associated endonuclease Cas9/Csn1, FANCF off-target5 non-target strand, FANCF off-target5 target strand, ... | Authors: | Pacesa, M, Jinek, M. | Deposit date: | 2022-01-10 | Release date: | 2022-10-26 | Last modified: | 2024-01-31 | Method: | X-RAY DIFFRACTION (2.4 Å) | Cite: | Structural basis for Cas9 off-target activity. Cell, 185, 2022
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7QR7
| SpCas9 bound to AAVS1 off-target2 DNA substrate | Descriptor: | AAVS1 off-target2 non-target strand, AAVS1 off-target2 target strand, AAVS1 sgRNA, ... | Authors: | Pacesa, M, JInek, M. | Deposit date: | 2022-01-10 | Release date: | 2022-10-26 | Last modified: | 2024-01-31 | Method: | X-RAY DIFFRACTION (3 Å) | Cite: | Structural basis for Cas9 off-target activity. Cell, 185, 2022
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7QQT
| SpCas9 bound to FANCF off-target1 DNA substrate | Descriptor: | CRISPR-associated endonuclease Cas9/Csn1, FANCF off-target1 non-target strand, FANCF off-target1 target strand, ... | Authors: | Pacesa, M, Jinek, M. | Deposit date: | 2022-01-10 | Release date: | 2022-10-26 | Last modified: | 2024-01-31 | Method: | X-RAY DIFFRACTION (2.5 Å) | Cite: | Structural basis for Cas9 off-target activity. Cell, 185, 2022
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7QR0
| SpCas9 bound to TRAC off-target1 DNA substrate | Descriptor: | CRISPR-associated endonuclease Cas9/Csn1, MAGNESIUM ION, POTASSIUM ION, ... | Authors: | Pacesa, M, Jinek, M. | Deposit date: | 2022-01-10 | Release date: | 2022-10-26 | Last modified: | 2024-01-31 | Method: | X-RAY DIFFRACTION (2.3 Å) | Cite: | Structural basis for Cas9 off-target activity. Cell, 185, 2022
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7QR1
| SpCas9 bound to TRAC off-target2 DNA substrate | Descriptor: | CRISPR-associated endonuclease Cas9/Csn1, MAGNESIUM ION, POTASSIUM ION, ... | Authors: | Pacesa, M, Jinek, M. | Deposit date: | 2022-01-10 | Release date: | 2022-10-26 | Last modified: | 2024-01-31 | Method: | X-RAY DIFFRACTION (2.6 Å) | Cite: | Structural basis for Cas9 off-target activity. Cell, 185, 2022
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7QQU
| SpCas9 bound to FANCF off-target2 DNA substrate | Descriptor: | CRISPR-associated endonuclease Cas9/Csn1, FANCF off-target2 non-target strand, FANCF off-target2 target strand, ... | Authors: | Pacesa, M, Jinek, M. | Deposit date: | 2022-01-10 | Release date: | 2022-10-26 | Last modified: | 2024-01-31 | Method: | X-RAY DIFFRACTION (2.45 Å) | Cite: | Structural basis for Cas9 off-target activity. Cell, 185, 2022
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7QQZ
| SpCas9 bound to FANCF off-target7 DNA substrate | Descriptor: | CRISPR-associated endonuclease Cas9/Csn1, FANCF off-target7 non-target strand, FANCF off-target7 target strand, ... | Authors: | Pacesa, M, Jinek, M. | Deposit date: | 2022-01-10 | Release date: | 2022-10-26 | Last modified: | 2024-01-31 | Method: | X-RAY DIFFRACTION (2.25 Å) | Cite: | Structural basis for Cas9 off-target activity. Cell, 185, 2022
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7P3B
| Human RNA ligase RTCB in complex with GMP and Co(II) | Descriptor: | ACETATE ION, COBALT (II) ION, FORMIC ACID, ... | Authors: | Kroupova, A, Ackle, F, Jinek, M. | Deposit date: | 2021-07-07 | Release date: | 2021-12-22 | Last modified: | 2024-01-31 | Method: | X-RAY DIFFRACTION (2.3 Å) | Cite: | Molecular architecture of the human tRNA ligase complex. Elife, 10, 2021
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3P3I
| Crystal structure of the F36A mutant of the fluoroacetyl-CoA-specific thioesterase FlK in complex with fluoroacetate and CoA | Descriptor: | COENZYME A, Fluoroacetyl coenzyme A thioesterase, fluoroacetic acid | Authors: | Weeks, A.M, Coyle, S.M, Jinek, M, Doudna, J.A, Chang, M.C.Y. | Deposit date: | 2010-10-04 | Release date: | 2010-10-20 | Last modified: | 2011-11-16 | Method: | X-RAY DIFFRACTION (2 Å) | Cite: | Structural and biochemical studies of a fluoroacetyl-CoA-specific thioesterase reveal a molecular basis for fluorine selectivity. Biochemistry, 49, 2010
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3P2Q
| Crystal structure of the fluoroacetyl-CoA-specific thioesterase, FlK | Descriptor: | Fluoroacetyl coenzyme A thioesterase | Authors: | Weeks, A.M, Coyle, S.M, Jinek, M, Doudna, J.A, Chang, M.C.Y. | Deposit date: | 2010-10-03 | Release date: | 2010-10-20 | Last modified: | 2011-11-16 | Method: | X-RAY DIFFRACTION (1.85 Å) | Cite: | Structural and biochemical studies of a fluoroacetyl-CoA-specific thioesterase reveal a molecular basis for fluorine selectivity. Biochemistry, 49, 2010
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3P3F
| Crystal structure of the F36A mutant of the fluoroacetyl-CoA-specific thioesterase FlK | Descriptor: | Fluoroacetyl coenzyme A thioesterase | Authors: | Weeks, A.M, Coyle, S.M, Jinek, M, Doudna, J.A, Chang, M.C.Y. | Deposit date: | 2010-10-04 | Release date: | 2010-10-20 | Last modified: | 2011-11-16 | Method: | X-RAY DIFFRACTION (2.3 Å) | Cite: | Structural and biochemical studies of a fluoroacetyl-CoA-specific thioesterase reveal a molecular basis for fluorine selectivity. Biochemistry, 49, 2010
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7P3A
| N-terminal domain of CGI-99 | Descriptor: | (4S)-2-METHYL-2,4-PENTANEDIOL, CHLORIDE ION, ISOPROPYL ALCOHOL, ... | Authors: | Kroupova, A, Jinek, M. | Deposit date: | 2021-07-07 | Release date: | 2021-12-22 | Method: | X-RAY DIFFRACTION (2 Å) | Cite: | Molecular architecture of the human tRNA ligase complex. Elife, 10, 2021
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5FQ5
| Crystal structure of Cas9-sgRNA-DNA complex solved by native SAD phasing | Descriptor: | CRISPR-ASSOCIATED ENDONUCLEASE CAS9/CSN1, MAGNESIUM ION, NON-TARGET DNA STRAND, ... | Authors: | Olieric, V, Weinert, T, Finke, A, Anders, C, Jinek, M, Wang, M. | Deposit date: | 2015-12-07 | Release date: | 2016-03-23 | Last modified: | 2024-05-08 | Method: | X-RAY DIFFRACTION (2.136 Å) | Cite: | Data-Collection Strategy for Challenging Native Sad Phasing. Acta Crystallogr.,Sect.D, 72, 2016
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