5WDT
 
 | | 70S ribosome-EF-Tu H84A complex with GppNHp | | Descriptor: | 16S rRNA, 23S rRNA, 30S ribosomal protein S10, ... | | Authors: | Fislage, M, Brown, Z, Frank, J. | | Deposit date: | 2017-07-06 | | Release date: | 2018-04-25 | | Last modified: | 2024-03-20 | | Method: | ELECTRON MICROSCOPY (3 Å) | | Cite: | Cryo-EM shows stages of initial codon selection on the ribosome by aa-tRNA in ternary complex with GTP and the GTPase-deficient EF-TuH84A.
Nucleic Acids Res., 46, 2018
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5WE6
 | | 70S ribosome-EF-Tu H84A complex with GTP and cognate tRNA | | Descriptor: | 16S rRNA, 23S rRNA, 30S ribosomal protein S10, ... | | Authors: | Fislage, M, Frank, J. | | Deposit date: | 2017-07-07 | | Release date: | 2018-04-25 | | Last modified: | 2024-03-13 | | Method: | ELECTRON MICROSCOPY (3.4 Å) | | Cite: | Cryo-EM shows stages of initial codon selection on the ribosome by aa-tRNA in ternary complex with GTP and the GTPase-deficient EF-TuH84A.
Nucleic Acids Res., 46, 2018
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8G38
 | | Time-resolved cryo-EM study of the 70S recycling by the HflX:3rd Intermediate | | Descriptor: | 16S, 23S, 30S ribosomal protein S10, ... | | Authors: | Bhattacharjee, S, Brown, P.Z, Frank, J. | | Deposit date: | 2023-02-07 | | Release date: | 2023-12-06 | | Last modified: | 2024-02-14 | | Method: | ELECTRON MICROSCOPY (3.2 Å) | | Cite: | Time resolution in cryo-EM using a PDMS-based microfluidic chip assembly and its application to the study of HflX-mediated ribosome recycling.
Cell, 187, 2024
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8G31
 | | Time-resolved cryo-EM study of the 70S recycling by the HflX:2nd Intermediate | | Descriptor: | 16S, 23S, 30S ribosomal protein S10, ... | | Authors: | Bhattacharjee, S, Brown, P.Z, Frank, J. | | Deposit date: | 2023-02-06 | | Release date: | 2023-12-06 | | Last modified: | 2024-02-14 | | Method: | ELECTRON MICROSCOPY (3.2 Å) | | Cite: | Time resolution in cryo-EM using a PDMS-based microfluidic chip assembly and its application to the study of HflX-mediated ribosome recycling.
Cell, 187, 2024
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8G2U
 | | Time-resolved cryo-EM study of the 70S recycling by the HflX:control-apo-70S at 900ms | | Descriptor: | 16S rRNA, 23S rRNA, 30S ribosomal protein S10, ... | | Authors: | Bhattacharjee, S, Brown, P.Z, Frank, J. | | Deposit date: | 2023-02-06 | | Release date: | 2023-12-06 | | Last modified: | 2024-02-14 | | Method: | ELECTRON MICROSCOPY (3 Å) | | Cite: | Time resolution in cryo-EM using a PDMS-based microfluidic chip assembly and its application to the study of HflX-mediated ribosome recycling.
Cell, 187, 2024
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8G34
 | | Time-resolved cryo-EM study of the 70S recycling by the HflX:1st intermediate | | Descriptor: | 16S, 23S, 30S ribosomal protein S10, ... | | Authors: | Bhattacharjee, S, Brown, P.Z, Frank, J. | | Deposit date: | 2023-02-06 | | Release date: | 2023-12-06 | | Last modified: | 2024-02-14 | | Method: | ELECTRON MICROSCOPY (3.2 Å) | | Cite: | Time resolution in cryo-EM using a PDMS-based microfluidic chip assembly and its application to the study of HflX-mediated ribosome recycling.
Cell, 187, 2024
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1JQS
 | | Fitting of L11 protein and elongation factor G (domain G' and V) in the cryo-em map of E. coli 70S ribosome bound with EF-G and GMPPCP, a nonhydrolysable GTP analog | | Descriptor: | 50S Ribosomal protein L11, Elongation Factor G | | Authors: | Agrawal, R.K, Linde, J, Segupta, J, Nierhaus, K.H, Frank, J. | | Deposit date: | 2001-08-07 | | Release date: | 2001-09-07 | | Last modified: | 2024-02-07 | | Method: | ELECTRON MICROSCOPY (18 Å) | | Cite: | Localization of L11 protein on the ribosome and elucidation of its involvement in EF-G-dependent translocation.
J.Mol.Biol., 311, 2001
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6PV6
 | | Functional Pathways of Biomolecules Retrieved from Single-particle Snapshots | | Descriptor: | CALCIUM ION, Peptidyl-prolyl cis-trans isomerase FKBP1B, Ryanodine receptor 1, ... | | Authors: | Dashti, A, des Georges, A, Frank, J, Ourmazd, A. | | Deposit date: | 2019-07-19 | | Release date: | 2020-08-12 | | Last modified: | 2024-03-20 | | Method: | ELECTRON MICROSCOPY (4.5 Å) | | Cite: | Retrieving functional pathways of biomolecules from single-particle snapshots.
Nat Commun, 11, 2020
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3DNY
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1ZC8
 | | Coordinates of tmRNA, SmpB, EF-Tu and h44 fitted into Cryo-EM map of the 70S ribosome and tmRNA complex | | Descriptor: | Elongation factor Tu, H2 16S rRNA, H2b d mRNA, ... | | Authors: | Valle, M, Gillet, R, Kaur, S, Henne, A, Ramakrishnan, V, Frank, J. | | Deposit date: | 2005-04-11 | | Release date: | 2005-04-19 | | Last modified: | 2024-02-14 | | Method: | ELECTRON MICROSCOPY (13 Å) | | Cite: | Visualizing tmRNA Entry into a Stalled Ribosome
Science, 300, 2003
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5KBS
 | | Cryo-EM structure of GluA2-0xSTZ at 8.7 Angstrom resolution | | Descriptor: | 2-acetamido-2-deoxy-beta-D-glucopyranose, Glutamate receptor 2,Voltage-dependent calcium channel gamma-2 subunit, {[7-morpholin-4-yl-2,3-dioxo-6-(trifluoromethyl)-3,4-dihydroquinoxalin-1(2H)-yl]methyl}phosphonic acid | | Authors: | Twomey, E.C, Yelshanskaya, M.V, Grassucci, R.A, Frank, J, Sobolevsky, A.I. | | Deposit date: | 2016-06-03 | | Release date: | 2016-07-13 | | Last modified: | 2020-07-29 | | Method: | ELECTRON MICROSCOPY (8.7 Å) | | Cite: | Elucidation of AMPA receptor-stargazin complexes by cryo-electron microscopy.
Science, 353, 2016
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5KBU
 | | Cryo-EM structure of GluA2-2xSTZ complex at 7.8 Angstrom resolution | | Descriptor: | 2-acetamido-2-deoxy-beta-D-glucopyranose, Glutamate receptor 2,Voltage-dependent calcium channel gamma-2 subunit, {[7-morpholin-4-yl-2,3-dioxo-6-(trifluoromethyl)-3,4-dihydroquinoxalin-1(2H)-yl]methyl}phosphonic acid | | Authors: | Twomey, E.C, Yelshanskaya, M.V, Grassucci, R.A, Frank, J, Sobolevsky, A.I. | | Deposit date: | 2016-06-03 | | Release date: | 2016-07-13 | | Last modified: | 2020-07-29 | | Method: | ELECTRON MICROSCOPY (7.8 Å) | | Cite: | Elucidation of AMPA receptor-stargazin complexes by cryo-electron microscopy.
Science, 353, 2016
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5KBV
 | | Cryo-EM structure of GluA2 bound to antagonist ZK200775 at 6.8 Angstrom resolution | | Descriptor: | 2-acetamido-2-deoxy-beta-D-glucopyranose, Glutamate receptor 2, {[7-morpholin-4-yl-2,3-dioxo-6-(trifluoromethyl)-3,4-dihydroquinoxalin-1(2H)-yl]methyl}phosphonic acid | | Authors: | Twomey, E.C, Yelshanskaya, M.V, Grassucci, R.G, Frank, J, Sobolevsky, A.I. | | Deposit date: | 2016-06-03 | | Release date: | 2016-07-13 | | Last modified: | 2020-07-29 | | Method: | ELECTRON MICROSCOPY (6.8 Å) | | Cite: | Elucidation of AMPA receptor-stargazin complexes by cryo-electron microscopy.
Science, 353, 2016
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5KBT
 | | Cryo-EM structure of GluA2-1xSTZ complex at 6.4 Angstrom resolution | | Descriptor: | 2-acetamido-2-deoxy-beta-D-glucopyranose, Glutamate receptor 2,Voltage-dependent calcium channel gamma-2 subunit, {[7-morpholin-4-yl-2,3-dioxo-6-(trifluoromethyl)-3,4-dihydroquinoxalin-1(2H)-yl]methyl}phosphonic acid | | Authors: | Twomey, E.C, Yelshanskaya, M.V, Grassucci, R.A, Frank, J, Sobolevsky, A.I. | | Deposit date: | 2016-06-03 | | Release date: | 2016-07-13 | | Last modified: | 2020-07-29 | | Method: | ELECTRON MICROSCOPY (6.4 Å) | | Cite: | Elucidation of AMPA receptor-stargazin complexes by cryo-electron microscopy.
Science, 353, 2016
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6ORL
 | | RF1 pre-accommodated 70S complex at 24 ms | | Descriptor: | 16S ribosomal RNA, 23S ribosomal RNA, 30S ribosomal protein S10, ... | | Authors: | Fu, Z, Indrisiunaite, G, Kaledhonkar, S, Shah, B, Sun, M, Chen, B, Grassucci, R.A, Ehrenberg, M, Frank, J. | | Deposit date: | 2019-04-30 | | Release date: | 2019-06-19 | | Last modified: | 2019-12-18 | | Method: | ELECTRON MICROSCOPY (3.5 Å) | | Cite: | The structural basis for release-factor activation during translation termination revealed by time-resolved cryogenic electron microscopy.
Nat Commun, 10, 2019
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6OT3
 | | RF2 accommodated state bound Release complex 70S at 24 ms | | Descriptor: | 16S ribosomal RNA, 23S ribosomal RNA, 30S ribosomal protein S10, ... | | Authors: | Fu, Z, Indrisiunaite, G, Kaledhonkar, S, Shah, B, Sun, M, Chen, B, Grassucci, R.A, Ehrenberg, M, Frank, J. | | Deposit date: | 2019-05-02 | | Release date: | 2019-06-19 | | Last modified: | 2019-12-18 | | Method: | ELECTRON MICROSCOPY (3.9 Å) | | Cite: | The structural basis for release-factor activation during translation termination revealed by time-resolved cryogenic electron microscopy.
Nat Commun, 10, 2019
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6OST
 | | RF2 pre-accommodated state bound Release complex 70S at 24ms | | Descriptor: | 16S Ribosomal RNA, 23S Ribosomal RNA, 30S ribosomal protein S10, ... | | Authors: | Fu, Z, Indrisiunaite, G, Kaledhonkar, S, Shah, B, Sun, M, Chen, B, Grassucci, R.A, Ehrenberg, M, Frank, J. | | Deposit date: | 2019-05-02 | | Release date: | 2019-06-19 | | Last modified: | 2019-12-18 | | Method: | ELECTRON MICROSCOPY (4.2 Å) | | Cite: | The structural basis for release-factor activation during translation termination revealed by time-resolved cryogenic electron microscopy.
Nat Commun, 10, 2019
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4C4Q
 | | Cryo-EM map of the CSFV IRES in complex with the small ribosomal 40S subunit and DHX29 | | Descriptor: | INTERNAL RIBOSOMAL ENTRY SITE | | Authors: | Hashem, Y, desGeorges, A, Dhote, V, Langlois, R, Liao, H.Y, Grassucci, R.A, Pestova, T.V, Hellen, C.U.T, Frank, J. | | Deposit date: | 2013-09-07 | | Release date: | 2013-10-30 | | Last modified: | 2024-05-08 | | Method: | ELECTRON MICROSCOPY (8.5 Å) | | Cite: | Hepatitis-C-Virus-Like Internal Ribosome Entry Sites Displace Eif3 to Gain Access to the 40S Subunit
Nature, 503, 2013
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1ZN0
 | | Coordinates of RRF and EF-G fitted into Cryo-EM map of the 50S subunit bound with both EF-G (GDPNP) and RRF | | Descriptor: | 16S RIBOSOMAL RNA, ELONGATION FACTOR G, Ribosome recycling factor | | Authors: | Gao, N, Zavialov, A.V, Li, W, Sengupta, J, Valle, M, Gursky, R.P, Ehrenberg, M, Frank, J. | | Deposit date: | 2005-05-11 | | Release date: | 2005-06-14 | | Last modified: | 2024-02-14 | | Method: | ELECTRON MICROSCOPY (15.5 Å) | | Cite: | Mechanism for the disassembly of the posttermination complex inferred from cryo-EM studies.
Mol.Cell, 18, 2005
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3DG5
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6XE0
 | | Cryo-EM structure of NusG-CTD bound to 70S ribosome (30S: NusG-CTD fragment) | | Descriptor: | 16s rRNA, 30S ribosomal protein S10, 30S ribosomal protein S11, ... | | Authors: | Washburn, R, Zuber, P, Sun, M, Hashem, Y, Shen, B, Li, W, Harvey, S, Acosta-Reyes, F.J, Knauer, S.H, Frank, J, Gottesman, M.E. | | Deposit date: | 2020-06-11 | | Release date: | 2020-07-29 | | Last modified: | 2024-03-06 | | Method: | ELECTRON MICROSCOPY (6.8 Å) | | Cite: | Escherichia coli NusG Links the Lead Ribosome with the Transcription Elongation Complex.
Iscience, 23, 2020
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3DG0
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1EG0
 | | FITTING OF COMPONENTS WITH KNOWN STRUCTURE INTO AN 11.5 A CRYO-EM MAP OF THE E.COLI 70S RIBOSOME | | Descriptor: | FORMYL-METHIONYL-TRNA, FRAGMENT OF 16S RRNA HELIX 23, FRAGMENT OF 23S RRNA, ... | | Authors: | Gabashvili, I.S, Agrawal, R.K, Spahn, C.M.T, Grassucci, R.A, Svergun, D.I, Frank, J, Penczek, P. | | Deposit date: | 2000-02-11 | | Release date: | 2000-03-06 | | Last modified: | 2024-02-07 | | Method: | ELECTRON MICROSCOPY (11.5 Å) | | Cite: | Solution structure of the E. coli 70S ribosome at 11.5 A resolution.
Cell(Cambridge,Mass.), 100, 2000
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5WEN
 | | GluA2 bound to GSG1L in digitonin, state 2 | | Descriptor: | Digitonin, Glutamate receptor 2,Germ cell-specific gene 1-like protein | | Authors: | Twomey, E.C, Yelshanskaya, M.V, Grassucci, R.A, Frank, J, Sobolevsky, A.I. | | Deposit date: | 2017-07-10 | | Release date: | 2017-08-02 | | Last modified: | 2021-08-25 | | Method: | ELECTRON MICROSCOPY (6.8 Å) | | Cite: | Channel opening and gating mechanism in AMPA-subtype glutamate receptors.
Nature, 549, 2017
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5WEO
 | | Activated GluA2 complex bound to glutamate, cyclothiazide, and STZ in digitonin | | Descriptor: | CYCLOTHIAZIDE, GLUTAMIC ACID, Glutamate receptor 2,Voltage-dependent calcium channel gamma-2 subunit chimera | | Authors: | Twomey, E.C, Yelshanskaya, M.V, Grassucci, R.A, Frank, J, Sobolevsky, A.I. | | Deposit date: | 2017-07-10 | | Release date: | 2017-08-02 | | Last modified: | 2019-11-20 | | Method: | ELECTRON MICROSCOPY (4.2 Å) | | Cite: | Channel opening and gating mechanism in AMPA-subtype glutamate receptors.
Nature, 549, 2017
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