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7ROF
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BU of 7rof by Molmil
Engineered tryptophan synthase b-subunit from Pyrococcus furiosus, PfTrpB2B9-H275E with L-Trp non-covalently bound
分子名称: SODIUM ION, TRYPTOPHAN, Tryptophan synthase beta chain 1
著者Higgins, P.M, Buller, A.R.
登録日2021-07-30
公開日2022-08-03
最終更新日2023-11-15
実験手法X-RAY DIFFRACTION (2.39 Å)
主引用文献Substrate multiplexed protein engineering facilitates promiscuous biocatalytic synthesis.
Nat Commun, 13, 2022
7RNP
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BU of 7rnp by Molmil
Engineered tryptophan synthase b-subunit from Pyrococcus furiosus, PfTrpB2B9_H275E with 4-Cl-Trp non-covalently bound
分子名称: 4-chloro-L-tryptophan, SODIUM ION, Tryptophan synthase beta chain 1
著者Higgins, P.M, Buller, A.R.
登録日2021-07-29
公開日2022-08-03
最終更新日2023-11-15
実験手法X-RAY DIFFRACTION (2.25 Å)
主引用文献Substrate multiplexed protein engineering facilitates promiscuous biocatalytic synthesis.
Nat Commun, 13, 2022
4WG2
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BU of 4wg2 by Molmil
P411BM3-CIS T438S I263F regioselective C-H amination catalyst
分子名称: Bifunctional P-450/NADPH-P450 reductase, PROTOPORPHYRIN IX CONTAINING FE, SULFATE ION
著者Hyster, T.K, Farwell, C.C, Buller, A.R, McIntosh, J.A, Arnold, F.H.
登録日2014-09-17
公開日2014-11-05
最終更新日2023-12-27
実験手法X-RAY DIFFRACTION (2.66 Å)
主引用文献Enzyme-controlled nitrogen-atom transfer enables regiodivergent C-h amination.
J.Am.Chem.Soc., 136, 2014
7N2T
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BU of 7n2t by Molmil
O-acetylserine sulfhydrylase from Citrullus vulgaris in the internal aldimine state, with citrate bound
分子名称: CITRIC ACID, Cysteine synthase, PENTAETHYLENE GLYCOL, ...
著者Smith, J.L, Buller, A.R, Bingman, C.A.
登録日2021-05-29
公開日2022-07-06
最終更新日2024-04-03
実験手法X-RAY DIFFRACTION (1.55 Å)
主引用文献Investigation of beta-Substitution Activity of O-Acetylserine Sulfhydrolase from Citrullus vulgaris.
Chembiochem, 23, 2022
7MKV
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BU of 7mkv by Molmil
Engineered PLP-dependent decarboxylative aldolase from Aspergillus flavus, UstD2.0, bound as the internal aldimine
分子名称: Cysteine desulfurase-like protein ustD
著者Ellis, J.M, Buller, A.R, Bingman, C.A.
登録日2021-04-27
公開日2022-05-04
実験手法X-RAY DIFFRACTION (2.25 Å)
主引用文献Biocatalytic synthesis of non-standard amino acids by a decarboxylative aldol reaction
Nat Catal, 5, 2022
6CUT
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BU of 6cut by Molmil
Engineered Holo TrpB from Pyrococcus furiosus, PfTrpB7E6 with (2S,3S)-isopropylserine bound as the external aldimine
分子名称: (2S,3S)-3-hydroxy-2-[(E)-({3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methylidene)amino]-4-methylpentanoic acid (non-preferred name), SODIUM ION, Tryptophan synthase beta chain 1
著者Boville, C.E, Scheele, R.A, Buller, A.R, Arnold, F.H.
登録日2018-03-26
公開日2018-09-26
最終更新日2024-03-13
実験手法X-RAY DIFFRACTION (1.77 Å)
主引用文献Engineered Biosynthesis of beta-Alkyl Tryptophan Analogues.
Angew. Chem. Int. Ed. Engl., 57, 2018
6CUV
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BU of 6cuv by Molmil
Engineered Holo TrpB from Pyrococcus furiosus, PfTrpB7E6
分子名称: PHOSPHATE ION, SODIUM ION, Tryptophan synthase beta chain 1
著者Scheele, R.A, Buller, A.R, Boville, C.E, Arnold, F.H.
登録日2018-03-26
公開日2018-09-26
最終更新日2023-11-15
実験手法X-RAY DIFFRACTION (2.26 Å)
主引用文献Engineered Biosynthesis of beta-Alkyl Tryptophan Analogues.
Angew. Chem. Int. Ed. Engl., 57, 2018
6CUN
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BU of 6cun by Molmil
Engineered Cytochrome c from Rhodothermus marinus (Rma TDE) bound to carbene intermediate (1-ethoxy-1-oxopropan-2-ylidene)
分子名称: Cytochrome c, ETHYL PROPIONATE, HEME C
著者Lewis, R.D, Buller, A.R, Arnold, F.H.
登録日2018-03-26
公開日2018-06-27
最終更新日2023-10-04
実験手法X-RAY DIFFRACTION (1.29 Å)
主引用文献Catalytic iron-carbene intermediate revealed in a cytochromeccarbene transferase.
Proc. Natl. Acad. Sci. U.S.A., 115, 2018
6CUK
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BU of 6cuk by Molmil
Engineered Cytochrome c from Rhodothermus marinus, Rma TDE
分子名称: Cytochrome c, HEME C
著者Lewis, R.D, Buller, A.R, Arnold, F.H.
登録日2018-03-26
公開日2018-06-27
最終更新日2023-10-04
実験手法X-RAY DIFFRACTION (1.47 Å)
主引用文献Catalytic iron-carbene intermediate revealed in a cytochromeccarbene transferase.
Proc. Natl. Acad. Sci. U.S.A., 115, 2018
5E4R
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BU of 5e4r by Molmil
Crystal structure of domain-duplicated synthetic class II ketol-acid reductoisomerase 2Ia_KARI-DD
分子名称: 1,2-ETHANEDIOL, GLYCEROL, Ketol-acid reductoisomerase, ...
著者Cahn, J.K.B, Brinkmann-Chen, S, Buller, A.R, Arnold, F.H.
登録日2015-10-07
公開日2015-12-23
最終更新日2023-09-27
実験手法X-RAY DIFFRACTION (1.94 Å)
主引用文献Artificial domain duplication replicates evolutionary history of ketol-acid reductoisomerases.
Protein Sci., 25, 2016
5UCW
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BU of 5ucw by Molmil
Cytochrome P411 P-4 A82L A78V F263L amination catalyst
分子名称: NADPH-cytochrome P450 reductase 102A1V3, PROTOPORPHYRIN IX CONTAINING FE
著者Zhang, R.K, Buller, A.R, Arnold, F.H.
登録日2016-12-22
公開日2017-05-17
最終更新日2023-10-04
実験手法X-RAY DIFFRACTION (1.7 Å)
主引用文献Enantioselective, intermolecular benzylic C-H amination catalysed by an engineered iron-haem enzyme.
Nat Chem, 9, 2017
6OJD
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BU of 6ojd by Molmil
A high-resolution crystal structure of covalent complex of NocB thioesterase domain with fluorophosphonate nocardicin G analog
分子名称: (R)-[(S)-[(3S)-3-{[(2R)-2-amino-2-(4-hydroxyphenyl)acetyl]amino}-2-oxoazetidin-1-yl](4-hydroxyphenyl)methyl]methylphosphinic acid, CALCIUM ION, GLYCEROL, ...
著者Patel, K.D, Gulick, A.M.
登録日2019-04-11
公開日2019-09-11
最終更新日2023-10-11
実験手法X-RAY DIFFRACTION (1.99 Å)
主引用文献Structure of a bound peptide phosphonate reveals the mechanism of nocardicin bifunctional thioesterase epimerase-hydrolase half-reactions.
Nat Commun, 10, 2019
6OJC
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BU of 6ojc by Molmil
A high-resolution crystal structure of NocB thioesterase domain from Nocardicin cluster
分子名称: GLYCEROL, NocB, SULFATE ION
著者Patel, K.D, Gulick, A.M.
登録日2019-04-11
公開日2019-09-11
最終更新日2024-03-13
実験手法X-RAY DIFFRACTION (1.94 Å)
主引用文献Structure of a bound peptide phosphonate reveals the mechanism of nocardicin bifunctional thioesterase epimerase-hydrolase half-reactions.
Nat Commun, 10, 2019
4XIY
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BU of 4xiy by Molmil
Crystal structure of ketol-acid reductoisomerase from Azotobacter
分子名称: DI(HYDROXYETHYL)ETHER, FE (III) ION, Ketol-acid reductoisomerase, ...
著者Spatzal, T, Cahn, J.K.B, Wiig, J.A, Einsle, O, Hu, Y, Ribbe, M.W, Arnold, F.H.
登録日2015-01-07
公開日2015-04-22
最終更新日2023-09-27
実験手法X-RAY DIFFRACTION (2.5 Å)
主引用文献Cofactor specificity motifs and the induced fit mechanism in class I ketol-acid reductoisomerases.
Biochem.J., 468, 2015
4XDZ
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BU of 4xdz by Molmil
Holo structure of ketol-acid reductoisomerase from Ignisphaera aggregans
分子名称: 4-(2-HYDROXYETHYL)-1-PIPERAZINE ETHANESULFONIC ACID, GLYCEROL, Ketol-acid reductoisomerase, ...
著者Cahn, J.K.B, Brinkmann-Chen, S, Arnold, F.H.
登録日2014-12-20
公開日2015-04-22
最終更新日2023-09-27
実験手法X-RAY DIFFRACTION (1.15 Å)
主引用文献Cofactor specificity motifs and the induced fit mechanism in class I ketol-acid reductoisomerases.
Biochem.J., 468, 2015
4XDY
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BU of 4xdy by Molmil
Structure of NADH-preferring ketol-acid reductoisomerase from an uncultured archean
分子名称: 1,4-DIHYDRONICOTINAMIDE ADENINE DINUCLEOTIDE, GLYCEROL, Ketol-acid reductoisomerase, ...
著者Cahn, J.K.B, Brinkmann-Chen, S, Arnold, F.H.
登録日2014-12-20
公開日2015-04-22
最終更新日2023-09-27
実験手法X-RAY DIFFRACTION (1.535 Å)
主引用文献Cofactor specificity motifs and the induced fit mechanism in class I ketol-acid reductoisomerases.
Biochem.J., 468, 2015
4XEH
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BU of 4xeh by Molmil
Apo structure of KARI from Ignisphaera aggregans
分子名称: Ketol-acid reductoisomerase
著者Cahn, J.K.B, Brinkmann-Chen, S, Arnold, F.H.
登録日2014-12-23
公開日2015-04-22
最終更新日2023-09-27
実験手法X-RAY DIFFRACTION (1.391 Å)
主引用文献Cofactor specificity motifs and the induced fit mechanism in class I ketol-acid reductoisomerases.
Biochem.J., 468, 2015
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