1ZS2
 
 | | Amylosucrase Mutant E328Q in a ternary complex with sucrose and maltoheptaose | | Descriptor: | alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose, alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose, amylosucrase, ... | | Authors: | Skov, L.K, Mirza, O, Sprogoe, D, van der Veen, B.A, Remaud-Simeon, M, Albenne, C, Monsan, P, Gajhede, M. | | Deposit date: | 2005-05-23 | | Release date: | 2006-05-02 | | Last modified: | 2023-08-23 | | Method: | X-RAY DIFFRACTION (2.16 Å) | | Cite: | Crystal structure of the Glu328Gln mutant of Neisseria polysaccharea amylosucrase in complex with sucrose and maltoheptaose
BIOCATAL.BIOTRANSFOR., 24, 2006
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1S46
 | | Covalent intermediate of the E328Q amylosucrase mutant | | Descriptor: | amylosucrase, beta-D-glucopyranose | | Authors: | Jensen, M.H, Mirza, O, Albenne, C, Remaud-Simeon, M, Monsan, P, Gajhede, M, Skov, L.K. | | Deposit date: | 2004-01-15 | | Release date: | 2004-03-23 | | Last modified: | 2021-10-27 | | Method: | X-RAY DIFFRACTION (2.2 Å) | | Cite: | Crystal structure of the covalent intermediate of amylosucrase from Neisseria polysaccharea.
Biochemistry, 43, 2004
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1MW2
 | | Amylosucrase soaked with 100mM sucrose | | Descriptor: | 2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL, alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose, amylosucrase, ... | | Authors: | Skov, L.K, Mirza, O, Sprogoe, D, Dar, I, Remaud-Simeon, M, Albenne, C, Monsan, P, Gajhede, M. | | Deposit date: | 2002-09-27 | | Release date: | 2002-12-18 | | Last modified: | 2024-03-13 | | Method: | X-RAY DIFFRACTION (2.1 Å) | | Cite: | Oligosaccharide and Sucrose Complexes of Amylosucrase. STRUCTURAL IMPLICATIONS FOR THE POLYMERASE ACTIVITY
J.BIOL.CHEM., 277, 2002
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1MW3
 | | Amylosucrase soaked with 1M sucrose | | Descriptor: | 2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL, amylosucrase, beta-D-fructofuranose-(2-1)-alpha-D-glucopyranose | | Authors: | Skov, L.K, Mirza, O, Sprogoe, D, Dar, I, Remaud-Simeon, M, Albenne, C, Monsan, P, Gajhede, M. | | Deposit date: | 2002-09-27 | | Release date: | 2002-12-18 | | Last modified: | 2024-03-13 | | Method: | X-RAY DIFFRACTION (2 Å) | | Cite: | Oligosaccharide and Sucrose Complexes of Amylosucrase. STRUCTURAL IMPLICATIONS FOR THE POLYMERASE ACTIVITY
J.BIOL.CHEM., 277, 2002
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1MVY
 | | Amylosucrase mutant E328Q co-crystallized with maltoheptaose. | | Descriptor: | 2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL, alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose, alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose, ... | | Authors: | Skov, L.K, Mirza, O, Sprogoe, D, Dar, I, Remaud-Simeon, M, Albenne, C, Monsan, P, Gajhede, M. | | Deposit date: | 2002-09-27 | | Release date: | 2002-12-18 | | Last modified: | 2024-05-29 | | Method: | X-RAY DIFFRACTION (2 Å) | | Cite: | Oligosaccharide and Sucrose Complexes of Amylosucrase. STRUCTURAL IMPLICATIONS FOR THE POLYMERASE ACTIVITY
J.BIOL.CHEM., 277, 2002
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1MW0
 | | Amylosucrase mutant E328Q co-crystallized with maltoheptaose then soaked with maltoheptaose. | | Descriptor: | 2,3-DIHYDROXY-1,4-DITHIOBUTANE, alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose, amylosucrase, ... | | Authors: | Skov, L.K, Mirza, O, Sprogoe, D, Dar, I, Remaud-Simeon, M, Albenne, C, Monsan, P, Gajhede, M. | | Deposit date: | 2002-09-27 | | Release date: | 2002-12-18 | | Last modified: | 2024-11-06 | | Method: | X-RAY DIFFRACTION (2.01 Å) | | Cite: | Oligosaccharide and Sucrose Complexes of Amylosucrase. STRUCTURAL IMPLICATIONS FOR THE POLYMERASE ACTIVITY
J.BIOL.CHEM., 277, 2002
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1MW1
 | | Amylosucrase soaked with 14mM sucrose. | | Descriptor: | 2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL, amylosucrase, beta-D-fructofuranose-(2-1)-alpha-D-glucopyranose | | Authors: | Skov, L.K, Mirza, O, Sprogoe, D, Dar, I, Remaud-Simeon, M, Albenne, C, Monsan, P, Gajhede, M. | | Deposit date: | 2002-09-27 | | Release date: | 2002-12-18 | | Last modified: | 2024-03-13 | | Method: | X-RAY DIFFRACTION (2.1 Å) | | Cite: | Oligosaccharide and Sucrose Complexes of Amylosucrase. STRUCTURAL IMPLICATIONS FOR THE POLYMERASE ACTIVITY
J.BIOL.CHEM., 277, 2002
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5LFC
 | | Crystal structure of Leuconostoc citreum NRRL B-1299 N-terminally truncated dextransucrase DSR-M | | Descriptor: | CALCIUM ION, DsrV, GLYCEROL | | Authors: | Claverie, M, Cioci, G, Remaud-simeon, M, Moulis, C, Tranier, S. | | Deposit date: | 2016-07-01 | | Release date: | 2017-10-11 | | Last modified: | 2024-01-10 | | Method: | X-RAY DIFFRACTION (3.2 Å) | | Cite: | Investigations on the Determinants Responsible for Low Molar Mass Dextran Formation by DSR-M Dextransucrase
Acs Catalysis, 7, 2017
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1G5A
 | | AMYLOSUCRASE FROM NEISSERIA POLYSACCHAREA | | Descriptor: | 2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL, 4-(2-HYDROXYETHYL)-1-PIPERAZINE ETHANESULFONIC ACID, AMYLOSUCRASE, ... | | Authors: | Skov, L.K, Mirza, O, Henriksen, A, De Montalk, G.P, Remaud-Simeon, M, Sarcabal, P, Willemot, R.-M, Monsan, P, Gajhede, M. | | Deposit date: | 2000-10-31 | | Release date: | 2001-10-31 | | Last modified: | 2024-02-07 | | Method: | X-RAY DIFFRACTION (1.4 Å) | | Cite: | Amylosucrase, A Glucan-synthesizing Enzyme from the alpha-Amylase Family
J.Biol.Chem., 276, 2001
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4AYS
 | | The Structure of Amylosucrase from D. radiodurans | | Descriptor: | AMYLOSUCRASE | | Authors: | Skov, L.K, Pizzut, S, Remaud-Simeon, M, Gajhede, M, Mirza, O. | | Deposit date: | 2012-06-21 | | Release date: | 2013-09-11 | | Last modified: | 2023-12-20 | | Method: | X-RAY DIFFRACTION (3.15 Å) | | Cite: | The Structure of Amylosucrase from Deinococcus Radiodurans Has an Unusual Open Active-Site Topology
Acta Crystallogr.,Sect.F, 69, 2013
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2WPG
 | | Sucrose Hydrolase | | Descriptor: | AMYLOSUCRASE OR ALPHA AMYLASE | | Authors: | Champion, E, Remaud-Simeon, M, Skov, L.K, Kastrup, J.S, Gajhede, M, Mirza, O. | | Deposit date: | 2009-08-06 | | Release date: | 2009-11-24 | | Last modified: | 2023-12-20 | | Method: | X-RAY DIFFRACTION (1.9 Å) | | Cite: | The Apo Structure of Sucrose Hydrolase from Xanthomonas Campestris Pv. Campestris Shows an Open Active-Site Groove
Acta Crystallogr.,Sect.D, 65, 2009
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6HTV
 | | Crystal structure of Leuconostoc citreum NRRL B-1299 N-terminally truncated dextransucrase DSR-M in complex with isomaltotetraose | | Descriptor: | Alternansucrase, CALCIUM ION, alpha-D-glucopyranose-(1-6)-alpha-D-glucopyranose-(1-6)-alpha-D-glucopyranose-(1-6)-alpha-D-glucopyranose | | Authors: | Claverie, M, Cioci, G, Remaud-Simeon, M, Moulis, C, Lippens, G. | | Deposit date: | 2018-10-04 | | Release date: | 2019-09-11 | | Last modified: | 2024-01-24 | | Method: | X-RAY DIFFRACTION (3.9 Å) | | Cite: | Futile Encounter Engineering of the DSR-M Dextransucrase Modifies the Resulting Polymer Length.
Biochemistry, 58, 2019
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6HVG
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5NGY
 | | Crystal structure of Leuconostoc citreum NRRL B-1299 dextransucrase DSR-M | | Descriptor: | CALCIUM ION, DSR-M glucansucrase inactive mutant E715Q, PRASEODYMIUM ION, ... | | Authors: | Claverie, M, Cioci, G, Remaud-simeon, M, Moulis, C, Tranier, S, Vuillemin, M. | | Deposit date: | 2017-03-20 | | Release date: | 2017-11-01 | | Last modified: | 2024-01-17 | | Method: | X-RAY DIFFRACTION (3.7 Å) | | Cite: | Investigations on the Determinants Responsible for Low Molar Mass Dextran Formation by DSR-M Dextransucrase
Acs Catalysis, 2017
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5O8L
 | | Crystal structure of Leuconostoc citreum NRRL B-1299 N-terminally truncated dextransucrase DSR-M in complex with sucrose | | Descriptor: | Alternansucrase, CALCIUM ION, beta-D-fructofuranose-(2-1)-alpha-D-glucopyranose | | Authors: | Claverie, M, Cioci, G, Remaud-simeon, M, Moulis, C, Tranier, S. | | Deposit date: | 2017-06-13 | | Release date: | 2017-11-29 | | Last modified: | 2024-05-08 | | Method: | X-RAY DIFFRACTION (3.6 Å) | | Cite: | Investigations on the Determinants Responsible for Low Molar Mass Dextran Formation by DSR-M Dextransucrase
Acs Catalysis, 7, 2017
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4TTU
 | | N-terminally truncated dextransucrase DSR-E from Leuconostoc mesenteroides NRRL B-1299 in complex with isomaltotriose | | Descriptor: | CALCIUM ION, DI(HYDROXYETHYL)ETHER, Dextransucrase, ... | | Authors: | Brison, Y, Remaud-Simeon, M, Mourey, L, Tranier, S. | | Deposit date: | 2014-06-23 | | Release date: | 2015-07-29 | | Last modified: | 2023-12-20 | | Method: | X-RAY DIFFRACTION (2.18 Å) | | Cite: | Structural Insights into the Carbohydrate Binding Ability of an alpha-(12) Branching Sucrase from Glycoside Hydrolase Family 70.
J.Biol.Chem., 291, 2016
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4TVC
 | | N-terminally truncated dextransucrase DSR-E from Leuconostoc mesenteroides NRRL B-1299 in complex with gluco-oligosaccharides | | Descriptor: | CALCIUM ION, Dextransucrase, GLYCEROL, ... | | Authors: | Brison, Y, Remaud-Simeon, M, Mourey, L, Tranier, S. | | Deposit date: | 2014-06-26 | | Release date: | 2015-07-29 | | Last modified: | 2023-12-20 | | Method: | X-RAY DIFFRACTION (1.85 Å) | | Cite: | Structural Insights into the Carbohydrate Binding Ability of an alpha-(12) Branching Sucrase from Glycoside Hydrolase Family 70.
J.Biol.Chem., 291, 2016
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4TVD
 | | N-terminally truncated dextransucrase DSR-E from Leuconostoc mesenteroides NRRL B-1299 in complex with D-glucose | | Descriptor: | CALCIUM ION, DI(HYDROXYETHYL)ETHER, Dextransucrase, ... | | Authors: | Brison, Y, Remaud-Simeon, M, Mourey, L, Tranier, S. | | Deposit date: | 2014-06-26 | | Release date: | 2015-08-05 | | Last modified: | 2023-12-20 | | Method: | X-RAY DIFFRACTION (2.3 Å) | | Cite: | Structural Insights into the Carbohydrate Binding Ability of an alpha-(12) Branching Sucrase from Glycoside Hydrolase Family 70.
J.Biol.Chem., 291, 2016
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6SZI
 | | ASR Alternansucrase in complex with isomaltose | | Descriptor: | Alternansucrase, CALCIUM ION, alpha-D-glucopyranose-(1-6)-alpha-D-glucopyranose | | Authors: | Cioci, G, Molina, M, Moulis, C, Remaud-Simeon, M. | | Deposit date: | 2019-10-02 | | Release date: | 2020-06-17 | | Last modified: | 2024-01-24 | | Method: | X-RAY DIFFRACTION (3 Å) | | Cite: | A specific oligosaccharide-binding site in the alternansucrase catalytic domain mediates alternan elongation.
J.Biol.Chem., 295, 2020
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6T16
 | | ASR Alternansucrase in complex with panose | | Descriptor: | Alternansucrase, CALCIUM ION, alpha-D-glucopyranose, ... | | Authors: | Cioci, G, Molina, M, Moulis, C, Remaud-Simeon, M. | | Deposit date: | 2019-10-03 | | Release date: | 2020-06-17 | | Last modified: | 2024-01-24 | | Method: | X-RAY DIFFRACTION (3.1 Å) | | Cite: | A specific oligosaccharide-binding site in the alternansucrase catalytic domain mediates alternan elongation.
J.Biol.Chem., 295, 2020
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6SYQ
 | | ASR Alternansucrase in complex with isomaltotriose | | Descriptor: | Alternansucrase, CALCIUM ION, alpha-D-glucopyranose, ... | | Authors: | Cioci, G, Molina, M, Moulis, C, Remaud-Simeon, M. | | Deposit date: | 2019-09-30 | | Release date: | 2020-06-17 | | Last modified: | 2024-01-24 | | Method: | X-RAY DIFFRACTION (3 Å) | | Cite: | A specific oligosaccharide-binding site in the alternansucrase catalytic domain mediates alternan elongation.
J.Biol.Chem., 295, 2020
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6T18
 | | ASR Alternansucrase in complex with oligoalternan | | Descriptor: | Alternansucrase, CALCIUM ION, alpha-D-glucopyranose-(1-3)-alpha-D-glucopyranose-(1-6)-alpha-D-glucopyranose, ... | | Authors: | Cioci, G, Molina, M, Moulis, C, Remaud-Simeon, M. | | Deposit date: | 2019-10-03 | | Release date: | 2020-06-17 | | Last modified: | 2024-01-24 | | Method: | X-RAY DIFFRACTION (3.15 Å) | | Cite: | A specific oligosaccharide-binding site in the alternansucrase catalytic domain mediates alternan elongation.
J.Biol.Chem., 295, 2020
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6T1P
 | | ASR Alternansucrase in complex with isomaltononaose | | Descriptor: | Alternansucrase, CALCIUM ION, alpha-D-glucopyranose-(1-6)-alpha-D-glucopyranose-(1-6)-alpha-D-glucopyranose, ... | | Authors: | Cioci, G, Molina, M, Moulis, C, Remaud-Simeon, M. | | Deposit date: | 2019-10-04 | | Release date: | 2020-06-17 | | Last modified: | 2024-01-24 | | Method: | X-RAY DIFFRACTION (3.5 Å) | | Cite: | A specific oligosaccharide-binding site in the alternansucrase catalytic domain mediates alternan elongation.
J.Biol.Chem., 295, 2020
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4FLQ
 | | Crystal structure of Amylosucrase double mutant A289P-F290I from Neisseria polysaccharea. | | Descriptor: | 2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL, Amylosucrase, GLYCEROL, ... | | Authors: | Guerin, F, Champion, E, Moulis, C, Barbe, S, Tran, T.H, Morel, S, Descroix, K, Monsan, P, Mulard, L.A, Remaud-Simeon, M, Andre, I, Mourey, L, Tranier, S. | | Deposit date: | 2012-06-15 | | Release date: | 2012-10-31 | | Last modified: | 2023-09-13 | | Method: | X-RAY DIFFRACTION (2.5 Å) | | Cite: | Applying pairwise combinations of amino Acid mutations for sorting out highly efficient glucosylation tools for chemo-enzymatic synthesis of bacterial oligosaccharides.
J.Am.Chem.Soc., 134, 2012
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4FLO
 | | Crystal structure of Amylosucrase double mutant A289P-F290C from Neisseria polysaccharea | | Descriptor: | 2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL, Amylosucrase, GLYCEROL, ... | | Authors: | Guerin, F, Champion, E, Moulis, C, Barbe, S, Tran, T.H, Morel, S, Descroix, K, Monsan, P, Mulard, L.A, Remaud-Simeon, M, Andre, I, Mourey, L, Tranier, S. | | Deposit date: | 2012-06-15 | | Release date: | 2012-10-31 | | Last modified: | 2023-09-13 | | Method: | X-RAY DIFFRACTION (2.2 Å) | | Cite: | Applying pairwise combinations of amino Acid mutations for sorting out highly efficient glucosylation tools for chemo-enzymatic synthesis of bacterial oligosaccharides.
J.Am.Chem.Soc., 134, 2012
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