2FFL
Crystal Structure of Dicer from Giardia intestinalis
Summary for 2FFL
| Entry DOI | 10.2210/pdb2ffl/pdb |
| Descriptor | Dicer, MANGANESE (II) ION (3 entities in total) |
| Functional Keywords | rna interference; rnai; paz domain, translation, hydrolase |
| Biological source | Giardia intestinalis |
| Total number of polymer chains | 4 |
| Total formula weight | 332902.86 |
| Authors | Doudna, J.A.,MacRae, I.J.,Adams, P.D. (deposition date: 2005-12-19, release date: 2006-01-24, Last modification date: 2024-02-14) |
| Primary citation | MacRae, I.J.,Zhou, K.,Li, F.,Repic, A.,Brooks, A.N.,Cande, W.Z.,Adams, P.D.,Doudna, J.A. Structural Basis of Double-Stranded RNA Processing by Dicer Science, 311:195-198, 2006 Cited by PubMed Abstract: The specialized ribonuclease Dicer initiates RNA interference by cleaving double-stranded RNA (dsRNA) substrates into small fragments about 25 nucleotides in length. In the crystal structure of an intact Dicer enzyme, the PAZ domain, a module that binds the end of dsRNA, is separated from the two catalytic ribonuclease III (RNase III) domains by a flat, positively charged surface. The 65 angstrom distance between the PAZ and RNase III domains matches the length spanned by 25 base pairs of RNA. Thus, Dicer itself is a molecular ruler that recognizes dsRNA and cleaves a specified distance from the helical end. PubMed: 16410517DOI: 10.1126/science.1121638 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (3.33 Å) |
Structure validation
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