Summary Structural details Experimental details Functional details Sequence Neighbor History Downloads

2FFL

Crystal Structure of Dicer from Giardia intestinalis

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0000166	molecular_function	nucleotide binding
A	0003723	molecular_function	RNA binding
A	0004518	molecular_function	nuclease activity
A	0004519	molecular_function	endonuclease activity
A	0004525	molecular_function	ribonuclease III activity
A	0006396	biological_process	RNA processing
A	0016787	molecular_function	hydrolase activity
A	0031047	biological_process	regulatory ncRNA-mediated gene silencing
A	0046872	molecular_function	metal ion binding
B	0000166	molecular_function	nucleotide binding
B	0003723	molecular_function	RNA binding
B	0004518	molecular_function	nuclease activity
B	0004519	molecular_function	endonuclease activity
B	0004525	molecular_function	ribonuclease III activity
B	0006396	biological_process	RNA processing
B	0016787	molecular_function	hydrolase activity
B	0031047	biological_process	regulatory ncRNA-mediated gene silencing
B	0046872	molecular_function	metal ion binding
C	0000166	molecular_function	nucleotide binding
C	0003723	molecular_function	RNA binding
C	0004518	molecular_function	nuclease activity
C	0004519	molecular_function	endonuclease activity
C	0004525	molecular_function	ribonuclease III activity
C	0006396	biological_process	RNA processing
C	0016787	molecular_function	hydrolase activity
C	0031047	biological_process	regulatory ncRNA-mediated gene silencing
C	0046872	molecular_function	metal ion binding
D	0000166	molecular_function	nucleotide binding
D	0003723	molecular_function	RNA binding
D	0004518	molecular_function	nuclease activity
D	0004519	molecular_function	endonuclease activity
D	0004525	molecular_function	ribonuclease III activity
D	0006396	biological_process	RNA processing
D	0016787	molecular_function	hydrolase activity
D	0031047	biological_process	regulatory ncRNA-mediated gene silencing
D	0046872	molecular_function	metal ion binding

Functional Information from PDB Data

site_id	AC1
Number of Residues	4
Details	BINDING SITE FOR RESIDUE MN A 5001

Chain	Residue
A	GLU649
A	ASP653
A	ASP720
A	GLU723

site_id	AC2
Number of Residues	5
Details	BINDING SITE FOR RESIDUE MN A 5002

Chain	Residue
A	GLU336
A	ASP340
A	ASP404
A	GLU407
A	HOH5039

site_id	AC3
Number of Residues	3
Details	BINDING SITE FOR RESIDUE MN A 5003

Chain	Residue
A	ASP653
A	GLU684
A	GLU723

site_id	AC4
Number of Residues	2
Details	BINDING SITE FOR RESIDUE MN A 5004

Chain	Residue
A	GLU336
A	GLU673

site_id	AC5
Number of Residues	1
Details	BINDING SITE FOR RESIDUE MN A 5005

Chain	Residue
A	HIS92

site_id	AC6
Number of Residues	1
Details	BINDING SITE FOR RESIDUE MN A 5006

Chain	Residue
A	HIS513

site_id	AC7
Number of Residues	4
Details	BINDING SITE FOR RESIDUE MN B 5007

Chain	Residue
B	GLU649
B	ASP653
B	ASP720
B	GLU723

site_id	AC8
Number of Residues	4
Details	BINDING SITE FOR RESIDUE MN B 5008

Chain	Residue
B	GLU336
B	ASP404
B	GLU407
B	HOH5047

site_id	AC9
Number of Residues	2
Details	BINDING SITE FOR RESIDUE MN B 5009

Chain	Residue
B	ASP653
B	GLU684

site_id	BC1
Number of Residues	2
Details	BINDING SITE FOR RESIDUE MN B 5010

Chain	Residue
B	GLU336
B	GLU673

site_id	BC2
Number of Residues	1
Details	BINDING SITE FOR RESIDUE MN B 5011

Chain	Residue
B	HIS92

site_id	BC3
Number of Residues	4
Details	BINDING SITE FOR RESIDUE MN B 5012

Chain	Residue
A	ASP714
B	GLU491
B	ASP495
D	HIS463

site_id	BC4
Number of Residues	3
Details	BINDING SITE FOR RESIDUE MN A 5013

Chain	Residue
A	GLU491
A	ASP495
B	ASP714

site_id	BC5
Number of Residues	4
Details	BINDING SITE FOR RESIDUE MN C 5014

Chain	Residue
C	GLU649
C	ASP653
C	ASP720
C	GLU723

site_id	BC6
Number of Residues	4
Details	BINDING SITE FOR RESIDUE MN C 5015

Chain	Residue
C	GLU336
C	ASP340
C	ASP404
C	GLU407

site_id	BC7
Number of Residues	3
Details	BINDING SITE FOR RESIDUE MN C 5016

Chain	Residue
C	ASP653
C	GLU684
C	GLU723

site_id	BC8
Number of Residues	2
Details	BINDING SITE FOR RESIDUE MN C 5017

Chain	Residue
C	GLU336
C	GLU673

site_id	BC9
Number of Residues	1
Details	BINDING SITE FOR RESIDUE MN C 5018

Chain	Residue
C	HIS92

site_id	CC1
Number of Residues	4
Details	BINDING SITE FOR RESIDUE MN C 5019

Chain	Residue
A	HIS463
C	GLU491
C	ASP495
D	ASP714

site_id	CC2
Number of Residues	5
Details	BINDING SITE FOR RESIDUE MN D 5020

Chain	Residue
D	GLU649
D	ASP653
D	ASP720
D	GLU723
D	HOH5045

site_id	CC3
Number of Residues	3
Details	BINDING SITE FOR RESIDUE MN D 5021

Chain	Residue
D	GLU336
D	ASP404
D	GLU407

site_id	CC4
Number of Residues	4
Details	BINDING SITE FOR RESIDUE MN D 5022

Chain	Residue
D	ASP653
D	GLU684
D	GLU723
D	HOH5041

site_id	CC5
Number of Residues	3
Details	BINDING SITE FOR RESIDUE MN D 5023

Chain	Residue
D	GLU336
D	ASP404
D	GLU673

site_id	CC6
Number of Residues	1
Details	BINDING SITE FOR RESIDUE MN D 5024

Chain	Residue
D	HIS92

site_id	CC7
Number of Residues	3
Details	BINDING SITE FOR RESIDUE MN C 5025

Chain	Residue
C	ASP714
D	GLU491
D	ASP495

site_id	CC8
Number of Residues	2
Details	BINDING SITE FOR RESIDUE MN A 5026

Chain	Residue
A	ASN202
A	HIS204

site_id	CC9
Number of Residues	2
Details	BINDING SITE FOR RESIDUE MN A 5027

Chain	Residue
A	ARG334
A	HIS665

site_id	DC1
Number of Residues	2
Details	BINDING SITE FOR RESIDUE MN B 5028

Chain	Residue
B	ASN202
B	HIS204

site_id	DC2
Number of Residues	3
Details	BINDING SITE FOR RESIDUE MN A 5029

Chain	Residue
A	HIS668
A	PRO669
A	THR670

site_id	DC3
Number of Residues	4
Details	BINDING SITE FOR RESIDUE MN C 5030

Chain	Residue
C	HIS668
C	PRO669
C	THR670
C	LEU671

site_id	DC4
Number of Residues	2
Details	BINDING SITE FOR RESIDUE MN D 5031

Chain	Residue
D	HIS745
D	HOH5040

site_id	DC5
Number of Residues	4
Details	BINDING SITE FOR RESIDUE MN C 5032

Chain	Residue
C	PHE355
C	PRO356
C	ASP357
D	LYS716

site_id	DC6
Number of Residues	1
Details	BINDING SITE FOR RESIDUE MN A 5033

Chain	Residue
A	HIS708

site_id	DC7
Number of Residues	5
Details	BINDING SITE FOR RESIDUE MN A 5034

Chain	Residue
A	PHE355
A	PRO356
A	ASP357
A	ALA358
B	LYS716

site_id	DC8
Number of Residues	2
Details	BINDING SITE FOR RESIDUE MN D 5035

Chain	Residue
D	GLN614
D	HIS610

site_id	DC9
Number of Residues	2
Details	BINDING SITE FOR RESIDUE MN C 5036

Chain	Residue
C	ASN202
C	HIS204

site_id	EC1
Number of Residues	3
Details	BINDING SITE FOR RESIDUE MN B 5037

Chain	Residue
A	ASN373
A	GLU374
B	ASN373

site_id	EC2
Number of Residues	1
Details	BINDING SITE FOR RESIDUE MN D 5038

Chain	Residue
D	GLU744

site_id	EC3
Number of Residues	2
Details	BINDING SITE FOR RESIDUE MN B 5039

Chain	Residue
B	ASP340
B	LYS657

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	484
Details	Domain: {"description":"RNase III 2","evidences":[{"source":"PROSITE-ProRule","id":"PRU00177","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

site_id	SWS_FT_FI2
Number of Residues	24
Details	Binding site: {"evidences":[{"source":"PubMed","id":"16410517","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"17704568","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"2FFL","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"2QVW","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

		Sequence (fasta)
		PDBx/mmCIF
		PDBML format (no-atom)
		PDB format (full)
		Validation report (PDF)
		EDMap 2fo-fc (MTZ)