9ZGL

Ancestrally reconstructed acetolactate synthase - Ancestor N259 with bound ThDP and magnesium.

Summary for 9ZGL

• Entry DOI: 10.2210/pdb9zgl/pdb
• Descriptor: Ancestral acetolactate synthase, catabolic, THIAMINE DIPHOSPHATE, MAGNESIUM ION, ... (4 entities in total)
• Functional Keywords: thdp, ancestor, transferase
• Biological source: synthetic construct
• Total number of polymer chains: 4
• Total formula weight: 247886.57

Authors

Douw, A.,Guddat, L.W. (deposition date: 2025-12-03, release date: 2025-12-17, Last modification date: 2026-07-22)

• Primary citation: Douw, A.,Krco, S.,Bayaraa, T.,Foley, G.,Davis, S.J.,Paredes Trujillo, O.,Asser, L.,Sieber, V.,Boden, M.,Hine, D.,Guddat, L.,Schenk, G.; Engineering of Acetolactate Synthase for Isobutanol Production Using Ancestral Sequence Reconstruction; To Be Published,

Experimental method

X-RAY DIFFRACTION (2.99 Å)