9YMX

De novo initial transcribing RNA polymerase with 5-mer RNA AUCUA (RPitc5b)

Summary for 9YMX

• Entry DOI: 10.2210/pdb9ymx/pdb
• EMDB information: 73125
• Descriptor: DNA-directed RNA polymerase subunit beta, MAGNESIUM ION, ZINC ION, ... (12 entities in total)
• Functional Keywords: rna polymerase, initial transcribing complex, nucleotide addition, promoter escape, transcription
• Biological source: Escherichia coli; More
• Total number of polymer chains: 9
• Total formula weight: 522939.71

Authors

Mueller, A.U.,Darst, S.A. (deposition date: 2025-10-10, release date: 2026-04-29, Last modification date: 2026-05-13)

Primary citation

Mueller, A.U.,Darst, S.A.
Structural basis for multi-subunit DNA-dependent RNA polymerase catalytic activity.
Mol.Cell, 2026

PubMed Abstract: Multi-subunit DNA-dependent RNA polymerase (RNAP) is the central enzyme of transcription, yet its catalytic mechanism remains obscure because high-resolution structures of intermediates with native substrates are not available. We visualized E. coli RNAP on a promoter DNA template with nucleoside triphosphate substrates engaged in active RNA synthesis by cryo-electron microscopy. From this heterogeneous mixture, we determined five high-resolution structures of initial transcribing complexes, including a true Michaelis complex (MC) and a post-catalytic product complex (PC). The MC reveals key conformational transitions during catalysis. Waters in the MC and PC structures show striking overlap with those in corresponding S. cerevisiae RNA polymerase II (RNAPII) structures (see related paper by Li et al.), pointing to functional importance. Together, these results establish that RNAP catalyzes nucleotidyl transfer through a positional (entropic) mechanism, revealing structural determinants of the first step of gene expression.

PubMed: 42066755
DOI: 10.1016/j.molcel.2026.03.033

Experimental method

ELECTRON MICROSCOPY (2.7 Å)