9YGL

RCK Gating Ring from Kch in the closed conformation

Summary for 9YGL

• Entry DOI: 10.2210/pdb9ygl/pdb
• Related: 9YE0 9YFV
• EMDB information: 72932
• Descriptor: Voltage-gated potassium channel Kch (2 entities in total)
• Functional Keywords: ion channel gating ring, rck, potassium channel, membrane protein, transport protein
• Biological source: Escherichia coli; More
• Total number of polymer chains: 8
• Total formula weight: 262249.32

Authors

Morote-Costas, B.,Zhou, M. (deposition date: 2025-09-29, release date: 2026-09-30)

Primary citation

Morote-Costas, B.,Pan, Y.,Wang, L.,Bai, X.,Lockless, S.W.,Zhou, M.
Mechanism of Kch activation by Zn 2.
Pnas Nexus, 5:pgag284-pgag284, 2026

PubMed Abstract: has a single K channel gene, , that produces a K channel with six transmembrane segments and a cytosolic Regulate Conductance of K channels (RCK) domain. RCK domains are present in both eukaryotic and prokaryotic organisms and known to regulate ion channel activity upon binding of an ion or a nucleotide, however, how Kch is regulated remains unknown. Here, we show that Zn or Cu activates Kch via the RCK domain. Structural studies reveal that eight RCK domains assemble into a gating ring that can assume three conformations, closed, intermediate, and open, and that Zn promotes the open conformation by stabilizing the assembly interface. These results expand our knowledge on Kch and on RCK-mediated regulation of ion channels.

PubMed: 42729885
DOI: 10.1093/pnasnexus/pgag284

Experimental method

ELECTRON MICROSCOPY (2.88 Å)