9Y6V
attPsym bound large serine integrase and RDF complex in the dimeric state
Summary for 9Y6V
| Entry DOI | 10.2210/pdb9y6v/pdb |
| EMDB information | 47284 47286 47287 47288 47289 47290 72552 72632 |
| Descriptor | Resolvase homolog YokA,SPbeta prophage-derived uncharacterized protein YotN, attPsym, ZINC ION, ... (4 entities in total) |
| Functional Keywords | site-specific dna recombinase, large serine integrase, dna binding domains, recombinase, resolvase, zinc ribbon recombinase, recombination |
| Biological source | Spbetavirus SPbeta More |
| Total number of polymer chains | 6 |
| Total formula weight | 327661.21 |
| Authors | |
| Primary citation | Shin, H.,Pigli, Y.,Pena Reyes, T.,Fuller, J.R.,Olorunniji, F.J.,Rice, P.A. Structural basis of directionality control in large serine integrases bioRxiv, 2025 Cited by PubMed Abstract: Large serine integrases (LSIs) catalyze unidirectional site-specific DNA recombination reactions, yet those reactions are reversed by the presence of a cognate recombination directionality factor (RDF). Mechanistic understanding of directionality control has been hampered by a lack of structural information. Here, we use cryo-electron microscopy (cryo-EM) to determine the structures of six SPbeta integrase-DNA complexes along the integrative (-RDF) and excisive (+RDF) reaction pathways, at 4.16-7.18Å resolution. Our findings reveal how RDF-mediated repositioning of an integrase subdomain (1) dictates which pairs of DNA sites can be assembled into a synaptic complex to initiate recombination and (2) dictates which product complexes will be conformationally locked, preventing the back reaction. These mechanistic insights provide a conceptual framework for engineering efficient and versatile genome editing tools. PubMed: 39803483DOI: 10.1101/2025.01.03.631226 PDB entries with the same primary citation |
| Experimental method | ELECTRON MICROSCOPY (7.1 Å) |
Structure validation
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