9Y6J

Avermitilol synthase: Complex with Mg, inorganic pyrophosphate, and bicyclic tetralin-based tertiary amine

This is a non-PDB format compatible entry.

Summary for 9Y6J

• Entry DOI: 10.2210/pdb9y6j/pdb
• Descriptor: Avermitilol synthase, 1,2-ETHANEDIOL, (2S)-N,N-dimethyl-1,2,3,4-tetrahydronaphthalen-2-amine, ... (6 entities in total)
• Functional Keywords: biosynthesis, terpene, cyclization, lyase
• Biological source: Streptomyces avermitilis
• Total number of polymer chains: 1
• Total formula weight: 37384.87

Authors

Gaynes, M.N.,Christianson, D.W. (deposition date: 2025-09-08, release date: 2025-12-17, Last modification date: 2025-12-31)

Primary citation

Gaynes, M.N.,Osika, K.R.,Christianson, D.W.
Structure and Mechanism of Avermitilol Synthase, a Sesquiterpene Cyclase That Generates a Highly Strained 6-6-3 Tricyclic Alcohol.
Biochemistry, 64:4830-4840, 2025

PubMed Abstract: Avermitilol synthase from (SaAS) is a high-fidelity class I terpene cyclase that converts farnesyl diphosphate into a highly strained, 6-6-3 tricyclic sesquiterpene alcohol. The mechanism of avermitilol formation proceeds through a 10-3 bicyclic intermediate, bicyclogermacrene, which undergoes proton-initiated anti-Markovnikov addition to two separate C═C bonds in a transannulation mechanism that forms the 6-6-3 tricyclic skeleton, with quenching by water to yield avermitilol. Small amounts of a side product, viridifloral, result from Markovnikov addition to one of the reactive C═C bonds. Here, we present enzymological studies of SaAS to establish the substrate scope and metal ion dependence for catalysis, and we present crystal structures of SaAS complexed with a variety of ligands that partially mimic carbocation intermediates in catalysis. Interestingly, these structures show that two water molecules remain trapped in a polar crevice in the active site regardless of the ligand bound. Structure-activity relationships for site-specific mutants yield key insight into the catalytic importance of these trapped water molecules. Specifically, T215 normally hydrogen bonds with water molecule W1, but the T215V substitution breaks this hydrogen bond and causes W1 to shift by 1.3 Å to form a hydrogen bond with W300. Avermitilol generation is completely lost in this mutant, but the generation of viridifloral and another side product is enhanced. We conclude that the T215V substitution causes water molecule W1 to align for reaction with the tertiary and not the secondary carbon in the reactive C═C bond of bicyclogermacrene.

PubMed: 41326024
DOI: 10.1021/acs.biochem.5c00565

Experimental method

X-RAY DIFFRACTION (1.44 Å)