9Y31
Symmetry relaxed asymmetric structure of an expansion intermediate of Turnip crinkle virus
Summary for 9Y31
| Entry DOI | 10.2210/pdb9y31/pdb |
| EMDB information | 72430 |
| Descriptor | Capsid protein (1 entity in total) |
| Functional Keywords | icosahedral non-enveloped ssrna virus, expansion intermediate, viral protein |
| Biological source | Turnip crinkle virus |
| Total number of polymer chains | 3 |
| Total formula weight | 114512.82 |
| Authors | Venkatakrishnan, V.,Braet, S.,Ramesh, R.,Clawson, M.A.,Laremore, T.N.,Wong, S.M.,Anand, G.S. (deposition date: 2025-09-01, release date: 2025-11-12, Last modification date: 2025-12-24) |
| Primary citation | Braet, S.M.,Venkatakrishnan, V.,Ramesh, R.,Clawson, M.A.,Laremore, T.N.,Wong, S.M.,Anand, G.S. Asymmetric isopeptide bond steers directional genomic RNA egress from icosahedral virus. Sci Adv, 11:eady4104-eady4104, 2025 Cited by PubMed Abstract: Icosahedral RNA viruses rely upon essential asymmetries for directional genome egress into the host cell. How these asymmetric egress points are built into the quaternary assembly of the virion is unknown. Here, we capture the structure and dynamics of a partially expanded virus disassembly intermediate, poised to release its spring-loaded genomic RNA. The structure shows highly localized density of RNA underneath surface fivefold axes on one-half of the viral particle. This polarity in RNA distribution is associated with a unique interchain isopeptide bond (glutamic acid-lysine), which flanks conserved high-affinity RNA binding sites. This singular isopeptide bond at the asymmetric egress site confers an essential "loaded-die" feature that is critical for steering genomic RNA egress into the host cell for virus propagation. PubMed: 41385643DOI: 10.1126/sciadv.ady4104 PDB entries with the same primary citation |
| Experimental method | ELECTRON MICROSCOPY (3.62 Å) |
Structure validation
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