9X5G
Cryo-EM structure of quinary complex GA3-MtGID1b-MtDELLA1-SLY1-ASK1
This is a non-PDB format compatible entry.
Summary for 9X5G
| Entry DOI | 10.2210/pdb9x5g/pdb |
| EMDB information | 66587 |
| Descriptor | DELLA protein 1, Gibberellin receptor GID1c-like protein, F-box protein GID2, ... (5 entities in total) |
| Functional Keywords | ga, plant protein |
| Biological source | Medicago truncatula (barrel medic) More |
| Total number of polymer chains | 4 |
| Total formula weight | 140798.69 |
| Authors | Wan, L.H. (deposition date: 2025-10-13, release date: 2026-07-08, Last modification date: 2026-08-26) |
| Primary citation | Xue, C.,Zhang, S.,Wan, L.,Zhang, Z.,Zhang, C.,Chen, H.,Peng, K.,Wang, Y.,Hu, J.,Gao, X.,Du, J.,Fu, X.,Xu, S. Structure-guided reprogramming of DELLA turnover for a sustainable Green Revolution in rice. Nat Commun, 17:-, 2026 Cited by PubMed Abstract: The Green Revolution of the 1960s significantly boosted cereal yields but incurred substantial environmental costs due to overreliance on chemical fertilizer inputs; thus, future agricultural sustainability demands improved nitrogen-use efficiency (NUE). The semi-dwarf Green Revolution varieties (GRVs) characterized by elevated DELLA protein accumulation introduce a fundamental trade-off, increasing lodging resistance and harvest index at the expense of diminished biomass and grain productivity per plant, and require high nitrogen fertilizer inputs to achieve maximum yield potential under high planting density. Here we show that gibberellin (GA)-bound GA-INSENSITIVE DWARF1 (GID1) induces multi-level conformational changes in DELLA protein, affecting its N-terminal DELLA and VHYN/DPT/S and C-terminal VVLV and SAW motifs, thereby facilitating recognition by the SKP1-CULLIN-F-box (SCF) ubiquitin ligase complex and subsequent proteasomal degradation. We also performed structure-guided engineering of the rice SLENDER RICE1 (SLR1)-GID1-GID2 complex and created a series of dwarf alleles exhibiting a continuous spectrum of plant heights in elite cultivars. Notably, the dominant alleles slr1 and slr1 achieved superior yield and enhanced NUE over conventional sd1-containing GRVs. Reprogramming DELLA turnover thus enables to break the long-standing trade-off between high yield and fertilizer dependency, offering a strategy toward a more sustainable and productive Green Revolution. PubMed: 42393083DOI: 10.1038/s41467-026-75068-8 PDB entries with the same primary citation |
| Experimental method | ELECTRON MICROSCOPY (3.24 Å) |
Structure validation
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