9W68

Crystal structural of dsPETase05

Summary for 9W68

• Entry DOI: 10.2210/pdb9w68/pdb
• Descriptor: PETase (2 entities in total)
• Functional Keywords: polyethylene terephthalate, petase, plastic waste recovery, lyase
• Biological source: Bacteria Latreille et al. 1825
• Total number of polymer chains: 1
• Total formula weight: 28815.39

Authors

Wang, Y.,Xu, B. (deposition date: 2025-08-03, release date: 2026-07-08)

Primary citation

Zhang, L.,Zhou, X.,Yuan, Y.,Li, H.,Li, J.,Zhou, Y.,Du, S.,Wang, Z.,Han, Y.,Fan, X.,Han, D.,Wang, L.,Zhu, C.,Ye, S.,Wang, Y.,Xu, B.
Structural and functional characterization of dsPETase05 for the degradation of Polyethylene terephthalate.
Int.J.Biol.Macromol., 343:150252-150252, 2026

PubMed Abstract: The enzymatic degradation of polyethylene terephthalate (PET) represents a promising sustainable strategy to address global plastic pollution. Among various plastic-degrading enzymes, PETase has been one of the most extensively studied and widely applied. Discovering novel PETase variants and elucidating their structure-function relationships are essential for developing more efficient enzymes. Here, we studied dsPETase05, a deep-sea PET hydrolase that was previously reported, which exhibits significantly enhanced PET degradation activity compared to the wild-type Ideonella sakaiensis PETase (IsPETase). This improvement was corroborated by atomic force microscopy (AFM) analysis of PET surface erosion and high-performance liquid chromatography (HPLC) quantification of hydrolysis products. We heterologously expressed and biochemically characterized dsPETase05, and resolved its crystal structure at 1.93 Å resolution. Structural analysis revealed that dsPETase05 adopts the typical α/β-hydrolase architecture found in PET-degrading enzymes, with distinctive amino acid substitutions near the catalytic triad that may contribute to its elevated activity. Our findings highlight the potential of marine-derived PETase for enzymatic plastic waste remediation and expand the repertoire of biocatalysts available for sustainable PET degradation.

PubMed: 41548779
DOI: 10.1016/j.ijbiomac.2026.150252

Experimental method

X-RAY DIFFRACTION (1.93 Å)