9W4I

Cryo-EM structure of Enterovirus-D68 MO strain virus-like particle

Summary for 9W4I

• Entry DOI: 10.2210/pdb9w4i/pdb
• EMDB information: 65634
• Descriptor: Capsid protein VP1, Capsid protein VP0, Capsid protein VP3 (3 entities in total)
• Functional Keywords: enterovirus-d68, mo strain, viral structural proteins, virus like particle
• Biological source: enterovirus D68; More
• Total number of polymer chains: 3
• Total formula weight: 95050.35

Authors

Senpuku, K.,Hirose, M.,Ito, T.,Kato, T.,Yshioka, Y. (deposition date: 2025-07-31, release date: 2026-07-01)

Primary citation

Senpuku, K.,Kunishima, Y.,Hirose, M.,Karaki, T.,Taniguchi, K.,Kataoka-Nakamura, C.,Hirai, T.,Yasuda, K.,Kuroda, E.,Kato, T.,Ito, T.,Yoshioka, Y.
Comparative immunogenic and structural analysis of virus-like particle and inactivated whole-virion vaccines against enterovirus D68.
Mol Ther Nucleic Acids, 37:102957-102957, 2026

PubMed Abstract: Enterovirus D68 (EV-D68) primarily causes respiratory illnesses and has been implicated in acute flaccid myelitis. Although virus-like particle (VLP) and traditional inactivated whole-virion (IWV) vaccines have demonstrated efficacy in mice, their immunological differences remain undetermined. Here, we directly compared the immunogenic and structural properties of VLP and IWV vaccines derived from the same EV-D68 strain under identical conditions. Although VLP induced significantly lower levels of EV-D68-specific IgG than IWV, neutralizing antibody titers and protective effects against viral challenge were comparable between the two groups in mice. Passive transfer experiments in neonatal mice further confirmed protection against lethal infection for both vaccine groups. Notably, in contrast to the IWV vaccine, the VLP vaccine elicited antibodies that preferentially recognized a limited subset of epitopes. Cryo-electron microscopy analyses revealed that VLPs structurally resemble the native virus but display distinct features in regions corresponding to epitopes that show differential antibody reactivity between VLP and IWV vaccines. By integrating structural and immunological analyses, we established a mechanistic framework linking capsid architecture to vaccine-induced antibody specificity. These findings suggest that VLP is a promising EV-D68 vaccine antigen with distinct epitope recognition profiles driven by structural characteristics.

PubMed: 42293247
DOI: 10.1016/j.omtn.2026.102957

Experimental method

ELECTRON MICROSCOPY (2.57 Å)