9W39
Structure of human 26S proteasome complexed with midnolin, 19S proteasome with Ubl bound
This is a non-PDB format compatible entry.
Summary for 9W39
| Entry DOI | 10.2210/pdb9w39/pdb |
| EMDB information | 65595 |
| Descriptor | Maltose/maltodextrin-binding periplasmic protein,Midnolin, 26S proteasome non-ATPase regulatory subunit 4, 26S proteasome non-ATPase regulatory subunit 8, ... (23 entities in total) |
| Functional Keywords | proteasome, midnolin, hydrolase |
| Biological source | Escherichia coli K-12 More |
| Total number of polymer chains | 19 |
| Total formula weight | 928496.47 |
| Authors | |
| Primary citation | Zhu, C.,Qin, L.,Dai, Z.,Zuo, P.,Yang, A.,Zhong, L.,Lin, Z.,Liang, L. Structural dynamics of the midnolin-proteasome during ubiquitin-independent substrate turnover. Nat Commun, 17:-, 2026 Cited by PubMed Abstract: The 26S proteasome typically degrades proteins marked by ubiquitin chains. However, a distinct, ubiquitin-independent degradation pathway for nuclear proteins exists, mediated by the adaptor protein midnolin, yet its molecular mechanism remains poorly understood. Here, we present nine cryo-electron microscopy structures of the human 26S proteasome in complex with midnolin, which collectively delineate a near-complete catalytic cycle. Our structures reveal that midnolin binds to the proteasome via the RPN1 subunit by its C-terminal helix. Unexpectedly, its ubiquitin-like domain interacts with the RPN11 deubiquitinase in a non-catalytic role. This interaction positions the adjacent Catch domain, which is responsible for substrate binding, directly above the proteasomal entrance, potentially facilitating substrate entry into the proteasome. Furthermore, we observe four consecutive spiral staircase conformations of the AAA+ ATPase hexamer during substrate translocation. These findings provide insights into the mechanisms underlying ubiquitin-independent nuclear protein degradation and may help develop strategies for targeting nuclear proteins via direct proteasomal degradation. PubMed: 41896529DOI: 10.1038/s41467-026-71002-0 PDB entries with the same primary citation |
| Experimental method | ELECTRON MICROSCOPY (3.65 Å) |
Structure validation
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