9VTF

Structural basis for the inhibition of cystathionine-b-synthase by isoflurane

Summary for 9VTF

• Entry DOI: 10.2210/pdb9vtf/pdb
• Descriptor: Cystathionine beta-synthase, PROTOPORPHYRIN IX CONTAINING FE, (2R)-2-chloro-2-(difluoromethoxy)-1,1,1-trifluoroethane (3 entities in total)
• Functional Keywords: hydrogen sulfide (h2s) biosynthesis, metabolism of homocysteine, biosynthetic protein
• Biological source: Homo sapiens (human)
• Total number of polymer chains: 4
• Total formula weight: 256459.38

Authors

Xu, K. (deposition date: 2025-07-10, release date: 2026-07-15)

Primary citation

Xu, K.
Structural basis for the inhibition of cystathionine-b-synthase by isoflurane and its role in anaesthesia-induced social dysfunction in mice
British Journal of Anaesthesia, 3:746-758, 2025

PubMed Abstract: Anaesthesia has been shown to impair social functioning, but the underlying mechanisms remain largely unknown. The volatile anaesthetic isoflurane potentially disrupts the methionine cycle and trans-sulphuration pathway, contributing to social deficits. Cystathionine-β-synthase (CBS), a key enzyme in this pathway, might be targeted by isoflurane. We investigated the CBS-isoflurane interaction and its role in neuronal function and social behaviour.

PubMed: 39603853
DOI: 10.1016/j.bja.2024.09.023

Experimental method

X-RAY DIFFRACTION (3.03 Å)