9VKI

Oligosaccharide sulfotransferase

Summary for 9VKI

• Entry DOI: 10.2210/pdb9vki/pdb
• Descriptor: LPS sulfotransferase NodH (1 entity in total)
• Functional Keywords: oligosaccharide sulfotransferase, sugar binding protein
• Biological source: Saccharothrix tamanrassetensis
• Total number of polymer chains: 2
• Total formula weight: 59655.04

Authors

Cao, Y.,Cao, Y. (deposition date: 2025-06-23, release date: 2026-06-03, Last modification date: 2026-07-08)

Primary citation

Cao, Y.,Guo, S.,Piao, Y.,Zhang, W.,Sun, P.,Cui, L.,Deng, Z.,Zhang, Q.,Ding, W.
Sulfation in the Biosynthesis of the Heptadecaglycoside Antibiotic Saccharomicin A.
Jacs Au, 6:3405-3413, 2026

PubMed Abstract: Sulfation is a ubiquitous modification in glycobiology, yet its enzymology and biological significance in glycosylated natural products remain poorly understood. Saccharomicin A, a potent oligosaccharide antibiotic, carries 17 sugars including a unique sulfated fucose. Here, we report the identification of Sam10 as an unprecedented fucose-specific sulfotransferase through genetic studies, reconstitution, and structural analysis, establishing its role in saccharomicin sulfation. Comparative bioassays revealed that sulfation contributes to antibacterial potency, including activity against diverse multidrug-resistant pathogens. We also characterized Sam35 as an efficient adenylyl-sulfate kinase that boosts cellular 3'-phosphoadenosine-5'-phosphate sulfate (PAPS) supply. Our findings define the biochemical and structural basis of saccharomicin sulfation and provide enzymatic tools for engineering novel sulfated oligosaccharide antibiotics.

PubMed: 42358689
DOI: 10.1021/jacsau.6c00414

Experimental method

X-RAY DIFFRACTION (3 Å)