9VD9Summary for 9VD9
| Entry DOI | 10.2210/pdb9vd9/pdb |
| EMDB information | 39339 |
| Descriptor | 26S proteasome complex subunit SEM1, Serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A alpha isoform, Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform, ... (41 entities in total) |
| Functional Keywords | dss1, integrator, intac, transcription |
| Biological source | Homo sapiens (human) More |
| Total number of polymer chains | 38 |
| Total formula weight | 2065239.01 |
| Authors | |
| Primary citation | Xu, C.,Zhou, Q.X.,Zheng, H.,Song, A.,Zhao, W.Y.,Xu, T.T.,Xiong, Y.,Zhang, Y.J.,Huang, Z.,Xu, Y.,Cheng, J.,Chen, F.X. DSS1 is required for proper Integrator-PP2A function. Nat Commun, 16:6206-6206, 2025 Cited by PubMed Abstract: Integrator-PP2A (INTAC) is a highly modular complex orchestrating the transition of paused RNA polymerase II into productive elongation or promoter-proximal premature termination, with its loss resulting in transcription dysregulation and genome instability. Here, we identify human DSS1-a flexible 70-residue protein found in multiple functionally diverse complexes including the 26S proteasome-as an integral subunit of the INTAC backbone. Structural analysis of DSS1-INTAC, both alone and in association with paused polymerase, demonstrates intimate interactions between DSS1 and the INTAC backbone. We identify tryptophan 39 of DSS1 as being critical for interacting with INTAC and find that its mutation disrupts DSS1's interaction with INTAC, while maintaining DSS1's interaction with the proteasome. This substitution not only impairs INTAC-dependent transcriptional regulation, but also reveals that INTAC is DSS1's major chromatin-bound form. Together, our findings reveal a role for DSS1 in supporting the structure and regulatory functions of INTAC. PubMed: 40617815DOI: 10.1038/s41467-025-61257-4 PDB entries with the same primary citation |
| Experimental method | ELECTRON MICROSCOPY (4.6 Å) |
Structure validation
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