9UYQ

Cryo-EM structure of a class C GPCR (Class 2)

Summary for 9UYQ

• Entry DOI: 10.2210/pdb9uyq/pdb
• Related: 9LLZ 9LM0
• EMDB information: 64622
• Descriptor: Metabotropic glutamate receptor 6, PHOSPHOSERINE (2 entities in total)
• Functional Keywords: gpcr, membrane protein
• Biological source: Homo sapiens (human)
• Total number of polymer chains: 2
• Total formula weight: 191448.68

Authors

Lee, S.Y.,Yun, Y.,Ji, J.S.,Jeong, H.,Lee, H.H. (deposition date: 2025-05-15, release date: 2026-03-18, Last modification date: 2026-09-30)

Primary citation

Lee, S.Y.,Chang, C.T.,Yun, Y.,Ji, J.S.,Martemyanov, K.A.,Lee, H.H.
CryoEM structure of mGlu6 captures receptor activation prior to G protein coupling.
Nat Commun, 17:-, 2026

PubMed Abstract: The metabotropic glutamate receptor 6 (mGlu6) is essential for synaptic communication of rod photoreceptors, and mutations in mGlu6 lead to a blinding disorder. However, its structural organization remains unknown. Here, we present the structure of agonist-bound mGlu6, revealing an asymmetric dimer arrangement in the absence of a G protein. This indicates that agonist binding alone can induce the homodimeric receptor asymmetry in metabotropic glutamate receptors and structurally prime mGlu6 for activation by pre-organizing the transmembrane domain dimer interface for G protein binding. The structure also identifies noncanonical interactions between the cysteine-rich domain and extracellular loop 2, forming a unique interface that likely stabilizes the activation state. Mutational analyses of this interface reveal its role in maintaining rapid Gαo activation and surface targeting. The structure also permits mechanistic investigation of congenital stationary night blindness and reveals diverse effects of pathogenic mutations on surface trafficking, Gαo coupling, and activation dynamics, including unexpected gain-of-function. These results provide critical insight into the intermediate asymmetric structure of mGlu6 and offer a molecular framework for understanding the pathogenesis of inherited retinal disorders.

PubMed: 41803130
DOI: 10.1038/s41467-026-70436-w

Experimental method

ELECTRON MICROSCOPY (3.27 Å)