9US7
Crystal Structure of Hemophore HphA from A. baumannii with Cobalt Phthalocyanine
This is a non-PDB format compatible entry.
Summary for 9US7
| Entry DOI | 10.2210/pdb9us7/pdb |
| Descriptor | Transferrin-binding protein-like solute binding protein, Cobalt(II) phthalocyanine, SULFATE ION, ... (4 entities in total) |
| Functional Keywords | hemophore, phthalocyanine, beta barrel, heme substitution, metal transport |
| Biological source | Acinetobacter baumannii AB5075 |
| Total number of polymer chains | 1 |
| Total formula weight | 24888.63 |
| Authors | Nguyen, V.Q.,Sugimoto, H.,Shoji, O. (deposition date: 2025-05-01, release date: 2026-05-20, Last modification date: 2026-06-24) |
| Primary citation | Nguyen, V.Q.,Sugimoto, H.,Shoji, O. Photodynamic Inactivation of Hypervirulent Acinetobacter baumannii via a Trojan Horse Strategy Targeting Heme Acquisition Pathway. Acs Infect Dis., 12:1924-1935, 2026 Cited by PubMed Abstract: Multidrug-resistant (MDR) pathogens pose a major threat to global health, and novel therapeutics are urgently needed to combat the rapid evolution of antimicrobial resistance. is one of the notorious MDR pathogens associated with severe hospital- and community-acquired infections worldwide. Herein, we demonstrate a "Trojan horse" strategy that exploits the recently discovered bacterial heme acquisition protein HphA to selectively sterilize . We found that the hemophore HphA, secreted by virulent strains, can accommodate several synthetic metal complexes such as 5,15-diphenylporphyrin and phthalocyanine that are structurally distinct from heme. Given that the crystal structure of HphA bound to synthetic metal complexes closely resembles that of the heme-bound form, we employed HphA as a carrier for gallium photosensitizers to photoinactivate the hypervirulent and MDR AB5075 strain. Upon photoirradiation, HphA incorporating gallium tetraphenylporphyrin and gallium 5,15-diphenylporphyrin (Ga-DPP) achieved efficient bacterial elimination, while a bromo-substituted Ga-DPP derivative exhibited superior sterilization efficiency, achieving 99.999% bacterial killing. These findings demonstrate that the HphA-mediated heme uptake pathway of can be harnessed to selectively and efficiently deliver photosensitizers, enabling potent photosterilization of antibiotic-resistant . PubMed: 42179064DOI: 10.1021/acsinfecdis.6c00032 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (1.41 Å) |
Structure validation
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