9UCP

cryo-EM structure of M1 muscarinic acetylcholine receptor-alpha5 helix of G11 protein complex bound to iperoxo and nanobody Nb1B4

Summary for 9UCP

• Entry DOI: 10.2210/pdb9ucp/pdb
• EMDB information: 64050
• Descriptor: M1 muscarinic acetylcholine receptor, de novo design protein, Guanine nucleotide-binding protein subunit alpha-11, Nanobody Nb1B4, ... (4 entities in total)
• Functional Keywords: gpcr, active-state, nanobody, de novo protein, membrane protein/immune system, membrane protein-immune system complex
• Biological source: Homo sapiens (human); More
• Total number of polymer chains: 3
• Total formula weight: 66434.04

Authors

Zhang, X.,Gao, K.,Liu, X. (deposition date: 2025-04-05, release date: 2025-10-29, Last modification date: 2026-05-13)

Primary citation

Zhang, X.,Gao, K.,Nie, J.,Meng, H.,Sun, X.,Zhao, J.,Liu, X.
Extracellular nanobody screening using conformationally stable GPCR variants.
Proc.Natl.Acad.Sci.USA, 122:e2508879122-e2508879122, 2025

PubMed Abstract: G protein-coupled receptors (GPCRs) are prominent drug targets that have attracted intensive efforts in drug screening. Binding-based screening methods for GPCR ligands often require conformationally stable, purified receptors. However, obtaining large quantities of GPCRs in stable states, particularly with unoccupied extracellular ligand-binding pockets and especially in their active conformations, remains challenging due to the inherent dynamic nature of these receptors. To address this challenge, we propose a universal approach for stabilizing GPCRs in specific conformations. Using the M1 muscarinic acetylcholine receptor (M1R) as a model, we successfully stabilized M1R in its active conformation through de novo design of a fusion protein, and further demonstrated the generalizability of this strategy by applying it to other GPCRs. We screened a synthetic yeast display library of nanobodies against both the stabilized active-state and previously reported inactive-state M1R, identifying several nanobodies that specifically recognize each conformation. This method not only facilitates the stabilization of GPCRs in desired states but also provides valuable tools for developing more selective therapeutic agents, enhancing drug discovery efficiency and specificity.

PubMed: 41187083
DOI: 10.1073/pnas.2508879122

Experimental method

ELECTRON MICROSCOPY (2.88 Å)