9UCM

Cryo-EM structure of the human G6P transporter SLC37A2 in the apo state with a symmetric ER luminal-open conformation.

Summary for 9UCM

• Entry DOI: 10.2210/pdb9ucm/pdb
• EMDB information: 64048
• Descriptor: Glucose-6-phosphate exchanger SLC37A2 (1 entity in total)
• Functional Keywords: transporter, g6p, mfs, er membrane, membrane protein
• Biological source: Homo sapiens (human)
• Total number of polymer chains: 2
• Total formula weight: 108954.21

Authors

Sun, L.,Liu, X.,Lai, Q. (deposition date: 2025-04-04, release date: 2025-11-05, Last modification date: 2026-05-27)

Primary citation

Lai, Q.,Xu, M.,Gao, Y.,Yang, Z.,Sun, L.,Liu, X.
Structural basis of glucose-6-phosphate transport by human SLC37A2.
Nat.Struct.Mol.Biol., 33:112-122, 2026

PubMed Abstract: Glucose-6-phosphate (G6P) transporters are crucial for glucose metabolism by mediating G6P transport from the cytoplasm to endoplasmic reticulum (ER). However, their transport mechanisms remain poorly understood. Here, we elucidate the structural and functional basis of human solute carrier family 37 member 2 (SLC37A2), a G6P transporter implicated in metabolic regulation and macrophage inflammation. We show that SLC37A2 functions as a uniporter, facilitating G6P transport independent of inorganic phosphate gradients. Structures of SLC37A2 in the apo and G6P-bound states reveal a dimeric architecture. Both the ER luminal-open and cytosolic-open structures are captured, showing the structural dynamics during G6P transport. G6P is coordinated by SLC37A2 through interactions with its phosphate and hydroxyl groups. Furthermore, mapping mutations associated with glycogen storage disease type Ib onto SLC37A2 highlights residues essential for transport activity. Together, this work provides structural insights into G6P transport and establishes a framework for understanding related metabolic disorders.

PubMed: 41225050
DOI: 10.1038/s41594-025-01712-4

Experimental method

ELECTRON MICROSCOPY (3.2 Å)