9UAT
The structure of mCAT1 in complex with its substrate ornithine and the RBD of FrMLV.
Summary for 9UAT
| Entry DOI | 10.2210/pdb9uat/pdb |
| EMDB information | 63995 |
| Descriptor | High affinity cationic amino acid transporter 1, Surface protein, 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, ... (6 entities in total) |
| Functional Keywords | receptor, complex, membrane protein |
| Biological source | Mus musculus (house mouse) More |
| Total number of polymer chains | 2 |
| Total formula weight | 92178.18 |
| Authors | |
| Primary citation | Xia, L.,Lin, B.,Zou, R.,Wu, Y.,Xu, J.,Pan, Y.,Yuan, Y.,Li, S.,Yang, Y.,Chen, X. Structural insights into cationic amino acid transport and viral receptor engagement by CAT1. Nat Commun, 17:1108-1108, 2025 Cited by PubMed Abstract: Cationic amino acid transporter 1 (CAT1) transports cationic amino acids and plays pivotal roles in cancer proliferation, immune modulation, and nitric oxide metabolism. It also serves as the specific cellular receptor for certain murine leukemia viruses. Here, we report the cryo-electron microscopy (cryo-EM) structure of mammalian CAT1 in complex with its substrate ornithine and the receptor-binding domain (RBD) of Friend murine leukemia virus (FrMLV). CAT1 specifically recognizes the side-chain amino group of ornithine via residue S347 on transmembrane helix 8 (TM8), capturing the transporter in an inward-facing occluded conformation. Notably, the FrMLV RBD (frRBD) primarily engages the third extracellular loop (ECL3) of CAT1-a region marked by substantial species-specific variation that likely governs cross-species viral tropism. Together, our structural and biochemical results elucidate the molecular mechanism of substrate recognition and transport by mCAT1, and unveil the molecular basis for FrMLV receptor specificity. These findings provide a valuable framework for structure-based drug design targeting CAT1 in cancer and infectious diseases. PubMed: 41408058DOI: 10.1038/s41467-025-67704-6 PDB entries with the same primary citation |
| Experimental method | ELECTRON MICROSCOPY (3.65 Å) |
Structure validation
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