9UA2

Cryo-EM structure of PsCas9-sgRNA-dsDNA (30 nt) ternary complex

Summary for 9UA2

Entry DOI	10.2210/pdb9ua2/pdb
EMDB information	63976
Descriptor	CRISPR-associated endonuclease Cas9, RNA (131-MER), DNA (30-MER), ... (5 entities in total)
Functional Keywords	crispr-cas9, rna, dna, complex, rna binding protein
Biological source	Parasutterella secunda More
Total number of polymer chains	4
Total formula weight	225824.86
Authors	Shen, P.P.,Lu, X.Y.,Huang, J.-W.,Chen, C.-C.,Guo, R.-T. (deposition date: 2025-03-31, release date: 2025-07-30)
Primary citation	Shen, P.,Lu, X.,Wang, C.,Huang, J.W.,Min, J.,Luo, J.,Sun, Y.,Chen, C.C.,Guo, R.T. Structural and Mechanistic Investigations of a High-Fidelity Cas9 from Parasutterella secunda Acs Catalysis, 15:10109-10118, 2025 Cited by DOI: 10.1021/acscatal.5c02993 PDB entries with the same primary citation
Experimental method	ELECTRON MICROSCOPY (2.83 Å)

Structure validation