9TYV

Cryo-EM structure of human TMEM45B with a bound GM3 (18:1;O2/24:1)

Summary for 9TYV

Entry DOI	10.2210/pdb9tyv/pdb
EMDB information	56439
Descriptor	Transmembrane protein 45B, UNKNOWN LIGAND, Neu5Ac-alpha2->3Gal-beta1->4Glc-beta1->1'Cer(d18:1/24:1, ... (5 entities in total)
Functional Keywords	orphan transmembrane protein, membrane protein
Biological source	Homo sapiens (human)
Total number of polymer chains	4
Total formula weight	134549.84
Authors	Grieben, M.,Inderhees, J. (deposition date: 2026-01-21, release date: 2026-10-07)
Primary citation	Grieben, M.,Inderhees, J.,Ebersberger, N. Cryo-EM structure of human TMEM45B with a bound GM3 (18:1;O2/24:1). J.Struct.Biol., 218:108376-108376, 2026 Cited by PubMed Abstract: The orphan transmembrane protein 45B (TMEM45B) has been reported to be involved in mechanical pain hypersensitivity, antiviral processes, and cancer. The structure of human TMEM45B with bound monosialodihexosylganglioside (GM3, 18:1;O2/24:1), presented here, determined by single-particle cryo-electron microscopy (cryo-EM) to 2.8 Å, reveals a homotetrameric assembly of seven-transmembrane-helix protomers. The first six transmembrane helices from each protomer create a central hydrophobic tunnel that accommodates metal ions and the C24:1 fatty acid component of the ganglioside GM3. PubMed: 42785650 DOI: 10.1016/j.jsb.2026.108376 PDB entries with the same primary citation
Experimental method	ELECTRON MICROSCOPY (2.82 Å)

Structure validation