9TMP

Crystal Structure of Native Cytochrome b562 in complex with the synthetic anti-BRIL antibody BAG2.

Summary for 9TMP

Entry DOI	10.2210/pdb9tmp/pdb
Descriptor	Soluble cytochrome b562, anti-BRIL antibody - heavy chain, anti-BRIL antibody - light chain, ... (5 entities in total)
Functional Keywords	cytochrome, fab, bril, protein binding
Biological source	synthetic construct More
Total number of polymer chains	3
Total formula weight	62502.62
Authors	Cooper, B.F.,Isom, G.L. (deposition date: 2025-12-15, release date: 2026-03-25, Last modification date: 2026-04-01)
Primary citation	Cooper, B.F.,Isom, G.L. Optimized bacterial expression of a synthetic BRIL antibody. Acta Crystallogr.,Sect.F, 2026 Cited by PubMed Abstract: The use of monoclonal fragments antigen binding (Fabs) is a prevalent methodology facilitating protein structure determination via both crystallography and cryo-EM. The development of a synthetic Fab against the BRIL domain improved the accessibility of this approach, providing a general fiducial applicable to any protein of interest via the simple curation of a BRIL fusion protein. Here, we document the generation of a T7 Express ΔcybC strain allowing contaminant-free bacterial expression of the synthetic anti-BRIL Fab BAG2. We also report the crystal structure of BAG2 in complex with native cytochrome b, a complex arising from expression in canonical Escherichia coli strains. PubMed: 41842660 DOI: 10.1107/S2053230X26001548 PDB entries with the same primary citation
Experimental method	X-RAY DIFFRACTION (2.65 Å)

Structure validation